CAJ | 학술논문

目的探讨糖尿病肾病大鼠血、尿中性粒细胞明胶酶相关脂笼蛋白(neutrophil gelatinase associated lipocalin,ngal)的水平及复方861对其的影响.方法采用一次性腹腔注射链脲佐菌素溶液制备糖尿病大鼠模型,随机分为正常对照组(N)、糖尿病组(D)、糖尿病中药治疗组(T)[中药复方861合剂1.5g·(kg·d)-1灌胃],于实验第2、4、8、12周处死,应用酶联免疫吸附法检测血清ngal(sngal)、尿ngal浓度(ungal).结果糖尿病组较正常组2周起血、尿ngal明显升高(P＜0.01);治疗组较糖尿病组2周起尿ngal降低(P＜0.01),4周血ngal明显降低(P＜0.01).结论血、尿ngal可作为糖尿病大鼠肾损伤的早期标记物.复方861可降低糖尿病大鼠血、尿ngal浓度而起到肾保护作用.
목적 탐토당뇨병신병대서혈、뇨중성립세포명효매상관지롱단백(neutrophil gelatinase associated lipocalin,ngal)적수평급복방861대기적영향.방법 채용일차성복강주사련뇨좌균소용액제비당뇨병대서모형,수궤분위정상대조조(N)、당뇨병조(D)、당뇨병중약치료조(T)[중약복방861합제1.5g·(kg·d)-1관위],우실험제2、4、8、12주처사,응용매련면역흡부법검측혈청ngal(sngal)、뇨ngal농도(ungal).결과 당뇨병조교정상조2주기혈、뇨ngal명현승고(P＜0.01);치료조교당뇨병조2주기뇨ngal강저(P＜0.01),4주혈ngal명현강저(P＜0.01).결론 혈、뇨ngal가작위당뇨병대서신손상적조기표기물.복방861가강저당뇨병대서혈、뇨ngal농도이기도신보호작용.
Objectives To study the neutrophil gelatinase associated lipocalin (NGAL)in the serum and urine of diabetic rats and the efficacy of compound chinese? herbal medicine 861 (ccm) on NGAL.Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ),and were randomly divided into three groups:normal control group (n =24),d iabete group (n =24),ccm [1.5 g · (kg · d)-1] treatment group (n =24).The concentration of NGAL in serum(sngal) and urine(ungal) was measured by euzymelinked immunosorbent assay at the 2nd,4th,8th and 12th week of experiment.Results NGAL in serum,urine was increased significantly at the 2nd week in diabetic group;In the ccm group sngal and ungal were decreased at the 4th and 2th week respectively compared with the diabetes group.Conclusions NGAL in the serum,urine increases in the early time in diabetic rats and on the increase over time.Ccm can decrease ngal in serum,urine and alleviates the renal tubular ultramicrostructural injury to protect the kidney.