CAJ | 학술논문

目的了解维生素D受体(vitamin D receptor,VDR)基因起始密码子(FokⅠ位点)和基因系尾型同源盒基因(caudal homcobox gene,CDX2)位点多态性与肾癌的关系.方法应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragmentlength polymorphism,PCR-RFLP)技术和等位基因特异多重PCR(allele-specific multiplex-PCR)方法,分别检测维生素D受体基因Fok Ⅰ和CDX2位点多态性等位基因及基因型在65例肾癌患者和85例健康对照者中的分布情况,并分析两组间基因型分布的差异.结果肾癌组VDR基因FokⅠ位点基因型FF 37.84％、Ff51.35％、ff10.81％,对照组分别为40％、40％、20％(x2=3.371,P＞0.05);肾癌组CDX2位点基因型GG 64.86％、AG 10.81％、AA 24.33％,健康人群组基因型分别为31.11％、18.89％、50％(x2=18.692,P＜0.01).结论维生素D受体基因Fok Ⅰ位点多态性可能与肾癌发病无关;CDX2位点多态性与肾癌发病可能相关.
목적 료해유생소D수체(vitamin D receptor,VDR)기인기시밀마자(FokⅠ위점)화기인계미형동원합기인(caudal homcobox gene,CDX2)위점다태성여신암적관계.방법 응용취합매련반응-한제성편단장도다태성(polymerase chain reaction-restriction fragmentlength polymorphism,PCR-RFLP)기술화등위기인특이다중PCR(allele-specific multiplex-PCR)방법,분별검측유생소D수체기인Fok Ⅰ화CDX2위점다태성등위기인급기인형재65례신암환자화85례건강대조자중적분포정황,병분석량조간기인형분포적차이.결과 신암조VDR기인FokⅠ위점기인형FF 37.84％、Ff51.35％、ff10.81％,대조조분별위40％、40％、20％(x2=3.371,P＞0.05);신암조CDX2위점기인형GG 64.86％、AG 10.81％、AA 24.33％,건강인군조기인형분별위31.11％、18.89％、50％(x2=18.692,P＜0.01).결론 유생소D수체기인Fok Ⅰ위점다태성가능여신암발병무관;CDX2위점다태성여신암발병가능상관.
Objectives To investigate the association of polymorphisms of start codon (Fok Ⅰ site) in vitamin D receptor gene (VDR) and CDX2 binding site concerned with the effect of kidney cancer.Methods The polymorphism? in VDR gene Fok Ⅰ,CDX2 were detected by polymerase chain reaction-restriction fragrnentlength polymorphism and allele-specific multiplex-PCR.Results Kidney cancer VDR gene FokⅠ of genotype was FF 37.84％,Ff 51.35％,FF 10.81％,the control group was 40％,40％,20％,(x2 =3.371,P ＞ 0.05).Kidney cancer group of the CDX2 genotype were GG 64.86％,AG 10.81％,AA 24.33％,group gene in healthy population were 31.11％,18.89％,50％ (x2 =18.692,P ＜0.01).Conclusions Vitamin D? receptor gene FokⅠ polymorphism may be not associated with the pathogenesis of kidney cancer;CDX2 polymorphism may be associated with kidney cancer.