中国实验诊断学
中國實驗診斷學
중국실험진단학
Chinese Journal of Laboratory Diagnosis
2015年
11期
1898-1900
,共3页
王平均%孙灵迪%梅传忠%武晓茜%邵先安
王平均%孫靈迪%梅傳忠%武曉茜%邵先安
왕평균%손령적%매전충%무효천%소선안
聚合酶链反应-直接测序法%磁珠酶联免疫法%人类白细胞抗原 B27%强直性脊柱炎
聚閤酶鏈反應-直接測序法%磁珠酶聯免疫法%人類白細胞抗原 B27%彊直性脊柱炎
취합매련반응-직접측서법%자주매련면역법%인류백세포항원 B27%강직성척주염
polymerase chain reaction sequence-based typing%immunomagnetic separation and enzyme-linked immu-nosorbent assay%human leukocyte antigen B27%ankylosing spondylitis
目的：探讨聚合酶链反应-直接测序法（polymerase chain reaction sequence-based typing，PCR-SBT）检测人类白细胞抗原 B27（human leukocyte antigen B27，HLA-B27）在强直性脊柱炎（ankylosing spondylitis，AS）临床诊断中的价值。方法应用 PCR-SBT 法和临床常用的 IMS-ELISA 法对120例疑似 AS 患者外周血进行 HLA-B27检测。以 SPSS17．0软件的配对χ2检验和受试者工作特征曲线下面积对两种方法检测 HLA-B27在 AS 诊断中的价值进行评价。结果120例疑似 AS 患者中，PCR-SBT 和 IMS-ELISA 法 HLA-B27检测阳性率分别为45．83％（55／120），37．50％（45／120）。两种方法检测 HLA-B27有统计学差异（P＝0．031）。PCR-SBT 法敏感度和特异度分别为96．36％，96．92％；IMS-ELISA 法的敏感度和特异度分别为69．09％，89．23％。两者的 AUC 分别为0．966和0．792。结论与传统的 IMS-ELISA 法相比，在 AS 的临床诊断中 PCR-SBT 法检测 HLA-B27的敏感度和特异度更高，方法更优。
目的：探討聚閤酶鏈反應-直接測序法（polymerase chain reaction sequence-based typing，PCR-SBT）檢測人類白細胞抗原 B27（human leukocyte antigen B27，HLA-B27）在彊直性脊柱炎（ankylosing spondylitis，AS）臨床診斷中的價值。方法應用 PCR-SBT 法和臨床常用的 IMS-ELISA 法對120例疑似 AS 患者外週血進行 HLA-B27檢測。以 SPSS17．0軟件的配對χ2檢驗和受試者工作特徵麯線下麵積對兩種方法檢測 HLA-B27在 AS 診斷中的價值進行評價。結果120例疑似 AS 患者中，PCR-SBT 和 IMS-ELISA 法 HLA-B27檢測暘性率分彆為45．83％（55／120），37．50％（45／120）。兩種方法檢測 HLA-B27有統計學差異（P＝0．031）。PCR-SBT 法敏感度和特異度分彆為96．36％，96．92％；IMS-ELISA 法的敏感度和特異度分彆為69．09％，89．23％。兩者的 AUC 分彆為0．966和0．792。結論與傳統的 IMS-ELISA 法相比，在 AS 的臨床診斷中 PCR-SBT 法檢測 HLA-B27的敏感度和特異度更高，方法更優。
목적：탐토취합매련반응-직접측서법（polymerase chain reaction sequence-based typing，PCR-SBT）검측인류백세포항원 B27（human leukocyte antigen B27，HLA-B27）재강직성척주염（ankylosing spondylitis，AS）림상진단중적개치。방법응용 PCR-SBT 법화림상상용적 IMS-ELISA 법대120례의사 AS 환자외주혈진행 HLA-B27검측。이 SPSS17．0연건적배대χ2검험화수시자공작특정곡선하면적대량충방법검측 HLA-B27재 AS 진단중적개치진행평개。결과120례의사 AS 환자중，PCR-SBT 화 IMS-ELISA 법 HLA-B27검측양성솔분별위45．83％（55／120），37．50％（45／120）。량충방법검측 HLA-B27유통계학차이（P＝0．031）。PCR-SBT 법민감도화특이도분별위96．36％，96．92％；IMS-ELISA 법적민감도화특이도분별위69．09％，89．23％。량자적 AUC 분별위0．966화0．792。결론여전통적 IMS-ELISA 법상비，재 AS 적림상진단중 PCR-SBT 법검측 HLA-B27적민감도화특이도경고，방법경우。
Objective The aim of this study was to compare PCR-SBT to IMS-ELISA in the HLA-B27 detection in the ankylosing spondylitis (AS)patients.Methods Simultaneously,PCR-SBT and IMS-ELISA were used to detect the HLA-B27 expression in peripheral blood samples which were suspected patients with AS from 120 cases.Chi square test of paired design and the area under curve of receiver operating characteristics of SPSS17.0 software were used to e-valuate the value of PCR-SBT and IMS-ELISA in HLA-B27 detection of AS patients.Results Among 120 cases of sus-pected patients with AS,the positive rates of HLA-B27 detected by PCR-SBT and IMS-ELISA were 45.83% (55/120),37.50%(45/120),respectively.There was statistical difference between the two methods in the HLA-B27 detec-tion (P =0.032).The sensibility and specificity of PCR-SBT were 96.36%,96.92%,respectively;while the sensibility and the specificity of IMS-ELISA were 69.09%,89.23%,respectively.Area under the curve of two methods were 0.966,0.792,separately.Conclusion In comparison with IMS-ELISA,the sensibility and the specificity of PCR-SBT in HLA-B27 detection were higher in AS diagnosis,that is to say,PCR-SBT is better in HLA-B27 detection and AS diag-nosis.
