CAJ | 학술논문

目的：探讨 Sirt1和 ProExC 在宫颈高度鳞状上皮内病变（HSIL）细胞学诊断中的价值。方法用免疫细胞化学技术检测436例液基细胞涂片（意义未明非典型鳞状上皮[ASCUS]213例，低级别鳞状上皮内病变[LSIL]101例，高级别鳞状上皮内病变[HSIL]122例）Sirt1和 ProEx C 表达情况；统计其在诊断宫颈 HSIL 的敏感度、特异度、准确度等评价指标。结果（1）Sirt1和 ProEx C 在预测 ASCUS 及 LSIL 中 HSIL 的敏感度分别为81．48％和74．07％，特异度分别为59．58％和71．43％，准确度分别为61．46％和71．65％，平行试验显示：Sirt1和 ProEx C 联合应用在预测 ASCUS 及 LSIL 中 HSIL 的敏感度为96．30％，特异度为42．51％。（2）Sirt1和 ProEx C 在诊断宫颈 HSIL 的敏感度分别为98．97％和100％，特异度为64％和76％，准确度为91．80％和95．08％，平行试验显示：两者联合应用其敏感度为100％，特异度为48．00％。结论Sirt1和 ProEx C 联合检测可提高宫颈 HSIL 细胞学诊断敏感度，降低漏诊率。
목적：탐토 Sirt1화 ProExC 재궁경고도린상상피내병변（HSIL）세포학진단중적개치。방법용면역세포화학기술검측436례액기세포도편（의의미명비전형린상상피[ASCUS]213례，저급별린상상피내병변[LSIL]101례，고급별린상상피내병변[HSIL]122례）Sirt1화 ProEx C 표체정황；통계기재진단궁경 HSIL 적민감도、특이도、준학도등평개지표。결과（1）Sirt1화 ProEx C 재예측 ASCUS 급 LSIL 중 HSIL 적민감도분별위81．48％화74．07％，특이도분별위59．58％화71．43％，준학도분별위61．46％화71．65％，평행시험현시：Sirt1화 ProEx C 연합응용재예측 ASCUS 급 LSIL 중 HSIL 적민감도위96．30％，특이도위42．51％。（2）Sirt1화 ProEx C 재진단궁경 HSIL 적민감도분별위98．97％화100％，특이도위64％화76％，준학도위91．80％화95．08％，평행시험현시：량자연합응용기민감도위100％，특이도위48．00％。결론Sirt1화 ProEx C 연합검측가제고궁경 HSIL 세포학진단민감도，강저루진솔。
Objective To study the Usefulness of Sirt1 and ProEx C in the cytologic diagnosis of cervical squamous intraepithelial lesions(SIL).Methods The expression of Sirt1 and ProEx C in 436 liquid-based cytologic samples(213 were ASCUS(atypical squamous cells of undetermined significance ),101 were LSIL(low-grade squamous intraepitheli-al lesion),122 were HSIL(high-grade squamous intraepithelial lesion))were done by immunocytochemistry,and their sensitivity,specificity and accuracy in the cytologic diagnosis of cervical HSIL were evaluated.Results ①The sensitivi-ty,specificity,accuracy of Sirt1 and ProEx C in predicting the HSIL in ASCUS and LSIL cytologic results were 81.48% and 74.07%,59.58% and 71.43%,61.46% and 71.65%,respectively;combined with Sirt1 and ProEx C in predicting the HSIL in ASCUS and LSIL cytologic results,the sensitivity was 96.30% and the specificity was 42.51%.②The sensitivity,specificity,accuracy of Sirt1 and ProEx C in diagnosis of HSIL were 98.97% and 100%, 64% and 76%,91.80% and 95.08%,respectively;combined with Sirt1 and ProEx C,the sensitivity was 100% and the specificity was 48.00%.Conclusion Sirt1 and ProEx C can improve the sensitivity in the cytologic diagnosis of HSIL.