中国癌症杂志
中國癌癥雜誌
중국암증잡지
China Oncology
2015年
9期
652-658
,共7页
周发忱%赵磊%白宇新%迟鑫明%周欣
週髮忱%趙磊%白宇新%遲鑫明%週訢
주발침%조뢰%백우신%지흠명%주흔
长链非编码RNA%HOTTIP%非小细胞肺癌%增殖%细胞凋亡
長鏈非編碼RNA%HOTTIP%非小細胞肺癌%增殖%細胞凋亡
장련비편마RNA%HOTTIP%비소세포폐암%증식%세포조망
lncRNA%HOTTIP%Non-small cell lung cancer%Proliferation%Apoptosis
背景与目的：探索非小细胞肺癌(non-small cell lung cancer，NSCLC)有效的早期诊断及预后标志物具有重要的科学意义和临床价值。长链非编码RNA(lncRNA)在肿瘤中的作用逐渐引起研究者的关注。本研究拟分析lncRNA HOTTIP在NSCLC中的表达情况及对NSCLC细胞增殖水平的影响和机制。方法：采用实时定量PCR(quantitative real-time PCR，qRT-PCR)检测方法检测54例NSCLC组织及其对应癌旁组织的HOTTIP表达情况，分析NSCLC组织HOTTIP表达与临床病理参数的相关性；MTT方法检测肺癌细胞增殖水平，流式细胞术检测凋亡率的改变；蛋白[质]印迹法(Western blot)检测目的基因的蛋白表达变化。结果：与癌旁组织比较，HOTTIP在肺癌组织中的表达率明显增加，差异有统计学意义(P<0.05)。HOTTIP表达与患者淋巴结转移和TNM分期相关。转染HOTTIP siRNA至A549肺癌细胞后，结果显示，与未转染组和阴性质粒转染组相比，转染siRNA-HOTTIP的A549细胞HOTTIP表达明显降低；HOTTIP表达下调后细胞增殖速度减慢，凋亡率增加，凋亡相关蛋白Bax的表达增加，Bcl-2表达减少。结论：HOTTIP是一种新的NSCLC生物标志物，可作为潜在的治疗新靶点。
揹景與目的：探索非小細胞肺癌(non-small cell lung cancer，NSCLC)有效的早期診斷及預後標誌物具有重要的科學意義和臨床價值。長鏈非編碼RNA(lncRNA)在腫瘤中的作用逐漸引起研究者的關註。本研究擬分析lncRNA HOTTIP在NSCLC中的錶達情況及對NSCLC細胞增殖水平的影響和機製。方法：採用實時定量PCR(quantitative real-time PCR，qRT-PCR)檢測方法檢測54例NSCLC組織及其對應癌徬組織的HOTTIP錶達情況，分析NSCLC組織HOTTIP錶達與臨床病理參數的相關性；MTT方法檢測肺癌細胞增殖水平，流式細胞術檢測凋亡率的改變；蛋白[質]印跡法(Western blot)檢測目的基因的蛋白錶達變化。結果：與癌徬組織比較，HOTTIP在肺癌組織中的錶達率明顯增加，差異有統計學意義(P<0.05)。HOTTIP錶達與患者淋巴結轉移和TNM分期相關。轉染HOTTIP siRNA至A549肺癌細胞後，結果顯示，與未轉染組和陰性質粒轉染組相比，轉染siRNA-HOTTIP的A549細胞HOTTIP錶達明顯降低；HOTTIP錶達下調後細胞增殖速度減慢，凋亡率增加，凋亡相關蛋白Bax的錶達增加，Bcl-2錶達減少。結論：HOTTIP是一種新的NSCLC生物標誌物，可作為潛在的治療新靶點。
배경여목적：탐색비소세포폐암(non-small cell lung cancer，NSCLC)유효적조기진단급예후표지물구유중요적과학의의화림상개치。장련비편마RNA(lncRNA)재종류중적작용축점인기연구자적관주。본연구의분석lncRNA HOTTIP재NSCLC중적표체정황급대NSCLC세포증식수평적영향화궤제。방법：채용실시정량PCR(quantitative real-time PCR，qRT-PCR)검측방법검측54례NSCLC조직급기대응암방조직적HOTTIP표체정황，분석NSCLC조직HOTTIP표체여림상병리삼수적상관성；MTT방법검측폐암세포증식수평，류식세포술검측조망솔적개변；단백[질]인적법(Western blot)검측목적기인적단백표체변화。결과：여암방조직비교，HOTTIP재폐암조직중적표체솔명현증가，차이유통계학의의(P<0.05)。HOTTIP표체여환자림파결전이화TNM분기상관。전염HOTTIP siRNA지A549폐암세포후，결과현시，여미전염조화음성질립전염조상비，전염siRNA-HOTTIP적A549세포HOTTIP표체명현강저；HOTTIP표체하조후세포증식속도감만，조망솔증가，조망상관단백Bax적표체증가，Bcl-2표체감소。결론：HOTTIP시일충신적NSCLC생물표지물，가작위잠재적치료신파점。
Background and purpose:Exploration of the effective early diagnostic and prognostic markers of non-small cell lung cancer (NSCLC) has important scientiifc signiifcance and clinical value. Recently, the role of long chain non-coding RNA (lncRNA) in the tumor attracts widespread attention. This study intended to investigate the level of lncRNA HOTTIP in NSCLC, the effect of HOTTIP on cell proliferation and its mechanisms.Methods:Expression of lncRNA HOTTIP in tumor and their matched non-tumor tissues were determined by quantitative real-time PCR (qRT-PCR) in NSCLC patients. Then, we analyzed the potential correlation of lncRNA HOTTIP expression levels in tumor tissues with clinicopathological features of NSCLC and clinical outcome. The effects of HOTTIP on NSCLC cell proliferation and apoptosis were tested usingin vitro MTT and lfow cytometric assays. Western blot method was uesd to detect the expressions of proteins.Results:LncRNA HOTTIP expression level was signiifcantly decreased in NSCLC tissues in comparison to adjacent non-tumor tissues (P<0.05). It was also proved that HOTTIP expression was associat-ed with NSCLC histological grade and lymph node metastasis. Moreover, knockdown of HOTTIP expression in A549 cell line decreased proliferation and enhanced apoptosis compared with transfected negative control. Western blot assay showed that the level of Bax protein was signiifcantly increased, whereas Bcl-2 was signiifcantly decreased in HOT-TIP-silencing A549 cell.Conclusion:HOTTIP is a novel prognostic biomarker and a potential therapeutic candidate forNSCLC.
