CAJ | 학술논문

目的：观察在人直肠癌组织中分化抑制因子‐2（Id‐2）和基质金属蛋白酶‐9（MMP‐9）的表达情况，分析两者表达水平的相关性，探讨直肠癌临床病理学指标与两者的表达水平的关系。方法收集直肠癌组织和癌旁正常组织标本56例，采用免疫组织化学方法观察Id‐2和MMP‐9在直肠癌和癌旁正常组织中的表达水平，Spearman 相关分析法分析Id‐2与MMP‐9表达有无相关性，分析Id‐2与MMP‐9表达与直肠癌临床病理学指标之间的关系。结果 Id‐2在直肠癌组织的阳性表达率明显高于癌旁正常组织（73．21％ vs ．48．21％，P＜0．05），MMP‐9在直肠癌组织中的阳性表达率高于癌旁正常组织（71．43％ vs ．44．64％，P＜0．05）。Spearman相关分析检测 Id‐2与 MMP‐9表达水平存在正相关性（r＝0．393，P＝0．003）；Id‐2和MMP‐9表达水平与直肠癌分化程度、TNM 分期和淋巴结转移有关（P＜0．05）；而与年龄和性别无关（P＞0．05）。结论 Id‐2和MMP‐9的表达水平与直肠癌的发生发展存在着密切的关系，直肠癌组织中Id‐2高表达可能是通过MMP‐9途径来促进肿瘤的侵袭转移。
목적：관찰재인직장암조직중분화억제인자‐2（Id‐2）화기질금속단백매‐9（MMP‐9）적표체정황，분석량자표체수평적상관성，탐토직장암림상병이학지표여량자적표체수평적관계。방법수집직장암조직화암방정상조직표본56례，채용면역조직화학방법관찰Id‐2화MMP‐9재직장암화암방정상조직중적표체수평，Spearman 상관분석법분석Id‐2여MMP‐9표체유무상관성，분석Id‐2여MMP‐9표체여직장암림상병이학지표지간적관계。결과 Id‐2재직장암조직적양성표체솔명현고우암방정상조직（73．21％ vs ．48．21％，P＜0．05），MMP‐9재직장암조직중적양성표체솔고우암방정상조직（71．43％ vs ．44．64％，P＜0．05）。Spearman상관분석검측 Id‐2여 MMP‐9표체수평존재정상관성（r＝0．393，P＝0．003）；Id‐2화MMP‐9표체수평여직장암분화정도、TNM 분기화림파결전이유관（P＜0．05）；이여년령화성별무관（P＞0．05）。결론 Id‐2화MMP‐9적표체수평여직장암적발생발전존재착밀절적관계，직장암조직중Id‐2고표체가능시통과MMP‐9도경래촉진종류적침습전이。
Objective To observe the expression level of inhibitor of differentiation 2 (Id‐2) and matrix metalloproteinases‐9 (MMP‐9) in rectal cancer ,analysis the correlation of the expression level of them ,to study the relationship between the expression level of them and the clinical pathology indicators of rectal cancer .Methods Rectal cancer tissues and normal tissue adjacent to rec‐tal cancer were obtained from the rectal cancer resection of 56 patients with rectal cancer ,using immunohistochemical method to ob‐serve the expression level of Id‐2 and MMP‐9 in normal tissue adjacent to rectal cancer and rectal cancer and Spearman correlation test to detect the correlation between the expression level of Id‐2 and MMP‐9 ;then we analyzed the relationships between the ex‐pression level of Id‐2 and MMP‐9 and the index of rectal cancer clinical pathology .Results The positive expression rate of Id‐2 in the in rectal cancer tissues is more higher than that of normal tissue of adjacent to rectal cancer (73 .21% vs .48 .21% ,P<0 .05) . The positive expression rate of MMP‐9 in the in rectal cancer tissues is higher than that of normal tissue of adjacent to rectal cancer (71 .43% vs .44 .64% ,P<0 .05) .Spearman correlation test showed that there is the positive correlation between the expression level of Id‐2 and MMP‐9 (r=0 .393 ,P=0 .003) .The expression levels of Id‐2 and MMP‐9 in rectal cancer were correlated with the degree of tumor differentiation ,TNM stage and lymph node metastasis (P<0 .05) ,but had no differences between the elements of age and sex (P>0 .05) .Conclusion There is a close relationship between the expression levels of Id‐2 and MMP‐9 in rectal cancer and the occurrence and development of rectal cancer .Rectal cancer with the higher Id‐2 expression level may be the ways to achieve tumor invasion and metastasis through MMP‐9 as a facilitator .