重庆医学
重慶醫學
중경의학
Chongqing Medicine
2015年
31期
4324-4326
,共3页
梁姣姣%居颂文%高志欣
樑姣姣%居頌文%高誌訢
량교교%거송문%고지흔
PD-L1%结肠癌%CD4+CD25hi CD127low/-T reg细胞
PD-L1%結腸癌%CD4+CD25hi CD127low/-T reg細胞
PD-L1%결장암%CD4+CD25hi CD127low/-T reg세포
PD-L1%colonic neoplasms%T-lymphocytes,regulatory%CD4+CD25hiCD127low/-
目的：探讨PD‐L1在结肠癌患者外周血CD4＋ CD25hi CD127low／‐T reg细胞上的表达及其与肿瘤病理分期之间的相关性。方法采用流式细胞术分析了47例结肠癌患者外周血中CD4＋CD25hi CD127low／‐T reg细胞的含量及其PD‐L1的表达。结果和健康人比较，结肠癌患者外周血中CD4＋CD25hi CD127low／‐T reg细胞的含量显著上升，并且PD‐L1的表达明显升高。进一步的分析表明，PD‐L1的表达和结肠癌的病理分期呈正相关，而术后患者CD4＋ CD25hi CD127low／‐T reg细胞 PD‐L1的表达则显著下降。结论 CD4＋CD25hi CD127low／‐T reg细胞数量及PD‐L1表达的升高可能导致了免疫抑制的增强，这可能是促进肿瘤免疫逃逸的重要因素。
目的：探討PD‐L1在結腸癌患者外週血CD4＋ CD25hi CD127low／‐T reg細胞上的錶達及其與腫瘤病理分期之間的相關性。方法採用流式細胞術分析瞭47例結腸癌患者外週血中CD4＋CD25hi CD127low／‐T reg細胞的含量及其PD‐L1的錶達。結果和健康人比較，結腸癌患者外週血中CD4＋CD25hi CD127low／‐T reg細胞的含量顯著上升，併且PD‐L1的錶達明顯升高。進一步的分析錶明，PD‐L1的錶達和結腸癌的病理分期呈正相關，而術後患者CD4＋ CD25hi CD127low／‐T reg細胞 PD‐L1的錶達則顯著下降。結論 CD4＋CD25hi CD127low／‐T reg細胞數量及PD‐L1錶達的升高可能導緻瞭免疫抑製的增彊，這可能是促進腫瘤免疫逃逸的重要因素。
목적：탐토PD‐L1재결장암환자외주혈CD4＋ CD25hi CD127low／‐T reg세포상적표체급기여종류병리분기지간적상관성。방법채용류식세포술분석료47례결장암환자외주혈중CD4＋CD25hi CD127low／‐T reg세포적함량급기PD‐L1적표체。결과화건강인비교，결장암환자외주혈중CD4＋CD25hi CD127low／‐T reg세포적함량현저상승，병차PD‐L1적표체명현승고。진일보적분석표명，PD‐L1적표체화결장암적병리분기정정상관，이술후환자CD4＋ CD25hi CD127low／‐T reg세포 PD‐L1적표체칙현저하강。결론 CD4＋CD25hi CD127low／‐T reg세포수량급PD‐L1표체적승고가능도치료면역억제적증강，저가능시촉진종류면역도일적중요인소。
Objective To investigate the expression of PD‐L1 in the CD4+CD25hiCD127low/‐ Treg cells in the peripheral blood of colon cancer and the correlation with clinical stage .Methods The expression of PD‐L1 on CD4+CD25hiCD127low/‐Treg cells from 47 samples of the peripheral blood of colon cancer was analyzed by flow cytometry .Results Compared with those from healthy do‐nor ,the proportion of CD4+CD25hiCD127low/‐Treg cells and expression of PD‐L1 on these cells increased significantly (P<0 .01) . Further analysis showed that expression of PD‐L1 increased with the increase of clinical stage of colon cancer (P<0 .01) ,and de‐creased significantly after postoperation (P<0 .05) .Conclusion Increase of proportion of CD4+CD25hiCD127low/‐Treg cells and the expression of PD‐L1 may enhance the suppression of antitumor immunity ,and thereby promote tumor immunoescape .
