CAJ | 학술논문

目的：研究胸腺素β4对人增生性瘢痕成纤维细胞中基质金属蛋白酶‐2（MMP‐2）和MMP‐9的表达影响，探讨胸腺素β4对增生性瘢痕的作用机制。方法2013年6～9月在南昌大学第一附属医院烧伤整形科手术切取的增生性瘢痕组织（烧伤后6个月内），体外培养成纤维细胞，分为实验组（胸腺素浓度分别为0．05、0．1、1．0、5．0μg／mL），对照组（不添加胸腺素β4），采用反转录‐聚合酶链反应（RT‐PCR）以及蛋白免疫印迹法（Western blot）测定实验组和对照组的MMP‐2、MMP‐9表达变化。结果胸腺素β4作用后增生性瘢痕组织内MMP‐2、MMP‐9水平增加，分别以1．0μg／mL实验组和5．0μg／mL实验组作用最明显，与对照组比较差异均有统计学意义（P＜0．05）。结论胸腺素β4能剂量依赖性促进MMP‐2、MMP‐9表达，可能是抑制增生性瘢痕的作用因素之一。
목적：연구흉선소β4대인증생성반흔성섬유세포중기질금속단백매‐2（MMP‐2）화MMP‐9적표체영향，탐토흉선소β4대증생성반흔적작용궤제。방법2013년6～9월재남창대학제일부속의원소상정형과수술절취적증생성반흔조직（소상후6개월내），체외배양성섬유세포，분위실험조（흉선소농도분별위0．05、0．1、1．0、5．0μg／mL），대조조（불첨가흉선소β4），채용반전록‐취합매련반응（RT‐PCR）이급단백면역인적법（Western blot）측정실험조화대조조적MMP‐2、MMP‐9표체변화。결과흉선소β4작용후증생성반흔조직내MMP‐2、MMP‐9수평증가，분별이1．0μg／mL실험조화5．0μg／mL실험조작용최명현，여대조조비교차이균유통계학의의（P＜0．05）。결론흉선소β4능제량의뢰성촉진MMP‐2、MMP‐9표체，가능시억제증생성반흔적작용인소지일。
Objective To study the effect of thymosin beta4 on the expression of matrix metalloproteinase‐2(MMP‐2) and MMP‐9 of human hypertrophic scar fibroblasts and explore mechanism of thymosin beta4 on hypertrophic scar .Methods Fibro‐blasts were cultured in vitro from the orthopaedic patients(within 6 months after burns) admitted in the first affiliated hospital of Nanchang university from June to September 2013 .The Fibroblasts were divided into experiment group :0 .05 ,0 .1 ,1 .0 μg/mL and 5 .0 μg/mL ,and control group :no thymosin beta4 .The expression levels of MMP‐2 ,MMP‐9 were observed by RT‐PCR and West‐ern blot .Results The expression levels of MMP‐2 and MMP‐9 were increased after the thymosin beta4 took effect ,and it was espe‐cially significant in 1 .0 μg/mL and 5 .0 μg/mL group ,there was statistical difference between experiment group and control group (P<0 .05) .Conclusion Thymosin beta4 can dose‐dependently promote the expression of MMP‐2 and MMP‐9 and inhibit the pro‐liferation of hypertrophic scar .