CAJ | 학술논문

目的：探索Ⅱ型胶原酶消化法分离兔肋软骨细胞的最佳消化时间。方法取3月龄新西兰大白兔肋软骨组织，采用0．2％Ⅱ型胶原酶分别消化4、8、12、16、20 h ，对应为A、B、C、D、E组，获取细胞后进行培养。通过比较5组P2代软骨细胞在形态、增殖速度、细胞内基质分泌等方面的差异，选择合适的消化时间。结果 C、D、E组获取的原代软骨细胞数量比A、B组多；C、D组P2代软骨细胞增殖能力强于其他3组；C、D组P2代细胞内基质分泌情况最佳。结论Ⅱ型胶原酶最佳消化时间为12～16 h ，获取的原代软骨细胞数量多，活性率较高，增殖能力强，P2代软骨细胞表型良好。
목적：탐색Ⅱ형효원매소화법분리토륵연골세포적최가소화시간。방법취3월령신서란대백토륵연골조직，채용0．2％Ⅱ형효원매분별소화4、8、12、16、20 h ，대응위A、B、C、D、E조，획취세포후진행배양。통과비교5조P2대연골세포재형태、증식속도、세포내기질분비등방면적차이，선택합괄적소화시간。결과 C、D、E조획취적원대연골세포수량비A、B조다；C、D조P2대연골세포증식능력강우기타3조；C、D조P2대세포내기질분비정황최가。결론Ⅱ형효원매최가소화시간위12～16 h ，획취적원대연골세포수량다，활성솔교고，증식능력강，P2대연골세포표형량호。
Objective To investigate the best digestion time for rabbit rib cartilage cells separation by collagenase Ⅱ diges‐tion method .Methods Rabbit cartilage tissue was taken from 3‐month‐old New Zealand white rabbits ,and then digested in 0 .2%collagenase Ⅱ solution for 4 ,8 ,12 ,16 and 20 h under vibration at 37 ℃ ,respectively .The corresponding experiments were named as group A ,B ,C ,D ,and E .The obtained primary cells were cultured and passaged after counting .The cell morphology ,proliferation rate ,secretion of extracellular matrix of passage 2 cells were compared to chose the best digestion time .Results The primary chon‐drocytes obtained in group C ,D and E were more than the other two group A and B .The chondrocytes cytoactive in group C and D were better than the other three group A ,B and E .The passage 2 chondrocytes in group C ,D had better secretion of extracellular matrix capability than the other three groups .Conclusion The best collagenase Ⅱ digestion time is 12 to 16 h ,at this digestion time period ,more primary chondrocytes with high cytoactive and strong proliferation capability can be obtained ,and the phenotype of their passage 2 cells is quite good .