国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
International Journal of Laboratory Medicine
2015年
22期
3265-3267,3270
,共4页
核酸检测%酶联免疫吸附法%化学发光
覈痠檢測%酶聯免疫吸附法%化學髮光
핵산검측%매련면역흡부법%화학발광
nucleic acid amplification technology%enzyme-linked immunosorbent assay%chemiluminescence method
目的：探讨核酸检测技术应用于献血者血液筛查的必要性和可行性。方法对2011年7月1日至2014年12月31日经ELISA检测均合格的献血者标本170316份，再采用核酸检测技术联合检测乙型肝炎病毒、丙型肝炎病毒、人类免疫缺陷病毒核酸，对筛查呈反应性的部分标本再送卫计委临床检验中心进行确证。结果共筛查出160份核酸反应性标本，总反应性率为0．09％，其中罗氏核酸检测系统反应性率为0．10％，达安核酸检测系统反应性率为0．08％，两者差异无统计学意义（ P＞0．05）。在27份送检标本中，有14份被确证为HBV DNA阳性，无HCV RNA和 HIV RNA的检出，确证阳性率为51．85％。有2份送检标本，采用化学发光法检测 HBsAg ，结果呈反应性。结论 ELISA方法筛查献血者标本存在漏检现象，核酸检测方法可作为ELISA的有效补充，能提高临床用血的安全性；化学发光法检测灵敏度优于ELSA。
目的：探討覈痠檢測技術應用于獻血者血液篩查的必要性和可行性。方法對2011年7月1日至2014年12月31日經ELISA檢測均閤格的獻血者標本170316份，再採用覈痠檢測技術聯閤檢測乙型肝炎病毒、丙型肝炎病毒、人類免疫缺陷病毒覈痠，對篩查呈反應性的部分標本再送衛計委臨床檢驗中心進行確證。結果共篩查齣160份覈痠反應性標本，總反應性率為0．09％，其中囉氏覈痠檢測繫統反應性率為0．10％，達安覈痠檢測繫統反應性率為0．08％，兩者差異無統計學意義（ P＞0．05）。在27份送檢標本中，有14份被確證為HBV DNA暘性，無HCV RNA和 HIV RNA的檢齣，確證暘性率為51．85％。有2份送檢標本，採用化學髮光法檢測 HBsAg ，結果呈反應性。結論 ELISA方法篩查獻血者標本存在漏檢現象，覈痠檢測方法可作為ELISA的有效補充，能提高臨床用血的安全性；化學髮光法檢測靈敏度優于ELSA。
목적：탐토핵산검측기술응용우헌혈자혈액사사적필요성화가행성。방법대2011년7월1일지2014년12월31일경ELISA검측균합격적헌혈자표본170316빈，재채용핵산검측기술연합검측을형간염병독、병형간염병독、인류면역결함병독핵산，대사사정반응성적부분표본재송위계위림상검험중심진행학증。결과공사사출160빈핵산반응성표본，총반응성솔위0．09％，기중라씨핵산검측계통반응성솔위0．10％，체안핵산검측계통반응성솔위0．08％，량자차이무통계학의의（ P＞0．05）。재27빈송검표본중，유14빈피학증위HBV DNA양성，무HCV RNA화 HIV RNA적검출，학증양성솔위51．85％。유2빈송검표본，채용화학발광법검측 HBsAg ，결과정반응성。결론 ELISA방법사사헌혈자표본존재루검현상，핵산검측방법가작위ELISA적유효보충，능제고림상용혈적안전성；화학발광법검측령민도우우ELSA。
Objective To evaluate the necessity and feasibility of nucleic acid test for donors blood screening .Methods From July 1 ,2011 to December 31 ,2014 ,a total of 170 316 blood samples which were negative in enzyme-linked immunosorbent assay (ELISA)and qualified in aianine aminotransferase detection ,were selected in this study stochastically .All the samples were detected hepatitis B virus(HBV) ,hepatitis C virus(HCV) ,human immunodeficiency virus(HIV) by nucleic acid amplification technology (NAT) .NAT positive samples were reconfirmed in National Center for Clinical Laboratories(NCCL) .Results A total of 160 cases of nucleic acid reactive samples were found out ,the total response rate was 0 .09% ,The response rate of Roche nucleic acid detec-tion system was 0 .10% ,response rate of David nucleic acid detection system was 0 .08% ,there was no significant difference be-tween the two methods(P>0 .05) .In 27 cases of specimens ,14 cases were confirmed as HBV DNA positive ,no HCV RNA and HIV RNA were detected ,the confirmed positive rate was 51 .85% .There were 2 samples detected by chemiluminescence HBsAg reactivity .Conclusion ELISA screening of blood donors has missing phenomenon ,nucleic acid detection method could be used as an effective supplement of the ELISA ,could improve the safety of blood for clinical use ,detection sensitivity is better than ELISA .
