CAJ | 학술논문

目的：评定血常规项目的测量不确定度，探讨室内质量控制（IQC ）数据结合参加希森美康网络通讯系统（SNCS ）的比对数据进行不确定评定方法的可行性。方法参照CNAS-TRL-001文件，对白细胞（WBC）计数、红细胞（RBC）计数、血红蛋白（Hb）测定、红细胞比积（HCT）、血小板计数（PLT）等5个血常规项目，采用IQC数据评定实验室内测量复现性引入的测量不确定度分量，分别采用能力验证（PT ）数据和SNCS数据评定偏移（bias）引入的标准不确定度，计算相对合成标准不确度及相对扩展不确定度，并比较PT数据和SNCS数据所得出的评定结果。结果各项目利用IQC 数据结合PT 数据评定的相对扩展不确定度，从水平1到水平3依次为：WBC（10．02％、7．24％、7．04％）；RBC（2．40％、1．72％、1．92％）；Hb（3．54％、2．56％、2．50％）；HCT（4．12％、3．18％、2．86％）；PLT（15．36％、8．86％、7．94％）。各项目利用IQC 数据结合SNCS比对数据评定的相对扩展不确定度，从水平1到水平3依次为WBC（11．66％、7．34％、6．40％）；RBC（2．26％、1．60％、1．64％）；Hb（3．36％、2．36％、2．10％）；HCT（3．36％、3．04％、3．18％）；PLT（13．34％、8．36％、7．14％）。结论血常规项目结果的测量不确定度符合其目标不确定度的要求，可以采用IQC数据结合SNCS数据进行血常规项目测量不确定度的评定。
목적：평정혈상규항목적측량불학정도，탐토실내질량공제（IQC ）수거결합삼가희삼미강망락통신계통（SNCS ）적비대수거진행불학정평정방법적가행성。방법삼조CNAS-TRL-001문건，대백세포（WBC）계수、홍세포（RBC）계수、혈홍단백（Hb）측정、홍세포비적（HCT）、혈소판계수（PLT）등5개혈상규항목，채용IQC수거평정실험실내측량복현성인입적측량불학정도분량，분별채용능력험증（PT ）수거화SNCS수거평정편이（bias）인입적표준불학정도，계산상대합성표준불학도급상대확전불학정도，병비교PT수거화SNCS수거소득출적평정결과。결과각항목이용IQC 수거결합PT 수거평정적상대확전불학정도，종수평1도수평3의차위：WBC（10．02％、7．24％、7．04％）；RBC（2．40％、1．72％、1．92％）；Hb（3．54％、2．56％、2．50％）；HCT（4．12％、3．18％、2．86％）；PLT（15．36％、8．86％、7．94％）。각항목이용IQC 수거결합SNCS비대수거평정적상대확전불학정도，종수평1도수평3의차위WBC（11．66％、7．34％、6．40％）；RBC（2．26％、1．60％、1．64％）；Hb（3．36％、2．36％、2．10％）；HCT（3．36％、3．04％、3．18％）；PLT（13．34％、8．36％、7．14％）。결론혈상규항목결과적측량불학정도부합기목표불학정도적요구，가이채용IQC수거결합SNCS수거진행혈상규항목측량불학정도적평정。
Objective To explore the feasibility of measurement uncertainty in complete blood count using internal quality con-trol(IQC) data associated with Sysmex Network Communication System(SNCS) comparative data .Methods Complete blood count assay including white blood cell(WBC) count ,red blood cell(RBC) count ,hemoglobin(Hb) determination ,hematocrit(HCT) values and platelet(PLT) count were involved to evaluate measurement uncertainty according to the instruction of CNAS-TRL-001 .Meas-urement uncertainty evaluation was established by internal measurement reproducibility using IQC data ,and standard measurement uncertainty by bias using proficiency testing(PT) data and SNCS data ,followed by relative combined standard measurement uncer-tainty and relative expanded measurement uncertainty was calculated .Meanwhile ,the measurement uncertainty was compared by u-sing PT data and SNAS comparative data .Results Relative expanded measurement uncertainty of the above mentioned index by u-sing IQC data associated with PT data was the following :WBC(10 .02% ,7 .24% ,7 .04% ,from level 1 to level 3 respectively) ,RBC (2 .40% ,1 .72% ,1 .92% ,from level 1 to level 3 respectively) ,Hb(3 .54% ,2 .56% ,2 .50% ,from level 1 to level 3 respectively) , HCT(4 .12% ,3 .18% ,2 .86% ,from level 1 to level 3 respectively) ,PLT(15 .36% ,8 .86% ,7 .94% ,from level 1 to level 3 respec-tively) .Relative expanded measurement uncertainty of the above mentioned index by using IQC data associated with SNCS compar-ative data was the following :WBC(11 .66% ,7 .34% ,6 .40% ,from level 1 to level 3 respectively) ,RBC(2 .26% ,1 .60% ,1 .64%from level 1 to level 3 respectively) ,Hb(3 .36% ,2 .36% ,2 .10% ,from level 1 to level 3 respectively) ,HCT (3 .36% ,3 .04% , 3 .18% ,from level 1 to level 3 respectively) ,PLT (13 .34% ,8 .36% ,7 .14% ,from level 1 to level 3 respectively) .Conclusion Measurement uncertainty in complete blood cell could be estimated by using IQC data associated with SNCS comparative data , which is in accord with the instrument of target measurement uncertainty .