CAJ | 학술논문

目的：探讨IL-18在BALB／c小鼠肺炎支原体（M P）感染模型肺泡灌洗液和血清中的表达，阐明其与M P感染的关系。方法72只BABL／c小鼠，随机分为3组，分别为对照组、M P感染组和治疗组，每组24只。用PPLO肉汤培养基培养M P ，将成功培养的MP滴入小鼠鼻腔建立MP感染模型。治疗组于模型建立的第5天使用克拉霉素治疗。MP感染模型建立后的第1、5、9天收集3组小鼠的眼球血及肺泡灌洗液，检测IL-18表达水平。分别于接种后3、7、14、21、30 d从3组中各取1只小鼠，完整分离肺组织，制作病理切片以观察MP感染小鼠肺的病变程度。结果病理切片显示，对照组小鼠肺组织无炎症反应，肺泡结构均匀清楚；MP感染组小鼠肺组织出现重度炎症改变；治疗组小鼠第7天的肺组织出现中度炎症改变。MP感染组及治疗组在建模后第1、5、9天时血清和肺泡灌洗液中IL-18表达水平较对照组均明显上升，差异有统计学意义（ t1天＝10．08，t5天＝9．14， t9天＝10．59，P＜0．05）。治疗组第5、9天血清和肺泡灌洗液中 IL-18表达水平均明显低于 M P感染组，差异均有统计学意义（t5天＝12．96，t9天＝7．95，P＜0．05）。结论 M P感染使小鼠IL-18水平降低，克拉霉素治疗有一定疗效，IL-18表达可能与M P肺炎严重程度有关。
목적：탐토IL-18재BALB／c소서폐염지원체（M P）감염모형폐포관세액화혈청중적표체，천명기여M P감염적관계。방법72지BABL／c소서，수궤분위3조，분별위대조조、M P감염조화치료조，매조24지。용PPLO육탕배양기배양M P ，장성공배양적MP적입소서비강건립MP감염모형。치료조우모형건립적제5천사용극랍매소치료。MP감염모형건립후적제1、5、9천수집3조소서적안구혈급폐포관세액，검측IL-18표체수평。분별우접충후3、7、14、21、30 d종3조중각취1지소서，완정분리폐조직，제작병리절편이관찰MP감염소서폐적병변정도。결과병리절편현시，대조조소서폐조직무염증반응，폐포결구균균청초；MP감염조소서폐조직출현중도염증개변；치료조소서제7천적폐조직출현중도염증개변。MP감염조급치료조재건모후제1、5、9천시혈청화폐포관세액중IL-18표체수평교대조조균명현상승，차이유통계학의의（ t1천＝10．08，t5천＝9．14， t9천＝10．59，P＜0．05）。치료조제5、9천혈청화폐포관세액중 IL-18표체수평균명현저우 M P감염조，차이균유통계학의의（t5천＝12．96，t9천＝7．95，P＜0．05）。결론 M P감염사소서IL-18수평강저，극랍매소치료유일정료효，IL-18표체가능여M P폐염엄중정도유관。
Objective To investigate the expression of interluekin(IL)-18 in BALB/c mice serum and bronchoalveolar fuid infec-ted by mycoplasma pneumonia(MP) and to clarify the relationship between IL-18 and MP infection .Methods A total of 72 BABL/c mice were randomly divided into control group ,MP infection group and treatment group ,each group with 24 mice .MP infection models were established with ripping cultured MP into nasal cavity of mice .Clarithromycin was used at the fifth day in the treat-ment group .The blood of eyeball and bronchoalveolar lavage fluid were collected at the first ,fifth ,ninth day in the three groups ,and detected IL-18 .At the third ,seventh ,forth ,twenty-first ,30th day ,one mice was selected to isolate lung tissue ,and make pathologi-cal section to observe lesion degree .Results Pathological section indicated that lung tissue of mice in the control group had no in-flammatory response ,but lung tissue in the M P infection group had serious inflammatory response ,lung tissue in the treatment group at seventh day had mild inflammatory response .Compared with that of the control group ,the IL-18 in the serum and bron-choalveolar lavage fluid significantly increased at the first ,fifth ,ninth day ,the differences were statistically significant (tfirst day =10 .08 ,tfifth day =9 .14 ,tninth day = 10 .59 ,P< 0 .05) .The IL-18 levels in the serum and bronchoalveolar lavage fluid at fifth day and ninth day in the treatment group were significantly lower than those of the MP infection group(tfifth day =12 .96 ,tninth day =17 .95 ,P<0 .05) .Conclusion MP infection causes IL-18 level increasing ,it is effective to treat with clarithromycin ,and IL-18 might correlate with pneumonia severity .