重庆医学
重慶醫學
중경의학
Chongqing Medicine
2015年
32期
4493-4495,4498
,共4页
王磊%胡晨曦%夏铀铀%宋大安%黎世秋%蒋晓东
王磊%鬍晨晞%夏鈾鈾%宋大安%黎世鞦%蔣曉東
왕뢰%호신희%하유유%송대안%려세추%장효동
胰腺肿瘤%PANC-1%干性细胞%放射敏感性
胰腺腫瘤%PANC-1%榦性細胞%放射敏感性
이선종류%PANC-1%간성세포%방사민감성
pancreatic neoplasms%PANC-1%stem cell%radiosensitive
目的：研究人胰腺癌PANC‐1细胞株中干性细胞放射敏感性，并探讨其放射抗拒的可能机制。方法应用流式细胞仪分选CD44＋CD24＋、CD44－CD24＋、CD44＋CD24－和CD44－CD24－细胞亚群；照射后，计算放射增敏比；利用流式细胞术检测凋亡及周期分布，并结合DCFH‐DA探针检查各亚群活性氧簇（ROS）水平。结果 PANC‐1细胞中CD44＋细胞比例约为92．0％， CD24＋细胞比例约为4．7％。照射前4组凋亡差异无统计学意义（ P＞0．05）；照射后CD44＋ CD24＋的凋亡比例最低（ P＜0．01）。照射前后CD44＋CD24＋的G0／G1期比例最高，显著高于其他3组（ P＜0．01）。照射后CD44＋ CD24－、CD44－CD24＋和CD44－CD24－放射增敏比分别为1．61、1．81、1．94；照射后CD44＋ CD24＋ ROS水平最低，平均荧光强度显著低于其他3组（ P＜0．01）。结论人胰腺癌PANC‐1细胞株中干性细胞大多处于静止期，且低ROS水平，因此考虑胰腺癌干性细胞的放射抗拒与此有关。
目的：研究人胰腺癌PANC‐1細胞株中榦性細胞放射敏感性，併探討其放射抗拒的可能機製。方法應用流式細胞儀分選CD44＋CD24＋、CD44－CD24＋、CD44＋CD24－和CD44－CD24－細胞亞群；照射後，計算放射增敏比；利用流式細胞術檢測凋亡及週期分佈，併結閤DCFH‐DA探針檢查各亞群活性氧簇（ROS）水平。結果 PANC‐1細胞中CD44＋細胞比例約為92．0％， CD24＋細胞比例約為4．7％。照射前4組凋亡差異無統計學意義（ P＞0．05）；照射後CD44＋ CD24＋的凋亡比例最低（ P＜0．01）。照射前後CD44＋CD24＋的G0／G1期比例最高，顯著高于其他3組（ P＜0．01）。照射後CD44＋ CD24－、CD44－CD24＋和CD44－CD24－放射增敏比分彆為1．61、1．81、1．94；照射後CD44＋ CD24＋ ROS水平最低，平均熒光彊度顯著低于其他3組（ P＜0．01）。結論人胰腺癌PANC‐1細胞株中榦性細胞大多處于靜止期，且低ROS水平，因此攷慮胰腺癌榦性細胞的放射抗拒與此有關。
목적：연구인이선암PANC‐1세포주중간성세포방사민감성，병탐토기방사항거적가능궤제。방법응용류식세포의분선CD44＋CD24＋、CD44－CD24＋、CD44＋CD24－화CD44－CD24－세포아군；조사후，계산방사증민비；이용류식세포술검측조망급주기분포，병결합DCFH‐DA탐침검사각아군활성양족（ROS）수평。결과 PANC‐1세포중CD44＋세포비례약위92．0％， CD24＋세포비례약위4．7％。조사전4조조망차이무통계학의의（ P＞0．05）；조사후CD44＋ CD24＋적조망비례최저（ P＜0．01）。조사전후CD44＋CD24＋적G0／G1기비례최고，현저고우기타3조（ P＜0．01）。조사후CD44＋ CD24－、CD44－CD24＋화CD44－CD24－방사증민비분별위1．61、1．81、1．94；조사후CD44＋ CD24＋ ROS수평최저，평균형광강도현저저우기타3조（ P＜0．01）。결론인이선암PANC‐1세포주중간성세포대다처우정지기，차저ROS수평，인차고필이선암간성세포적방사항거여차유관。
Objective To investigate the radiosensitivity of stem cells in pancreatic adenocarcinoma PANC‐1 cell line and the possible mechanism of its radiation resistance .Methods Using flow cytometry ,the cells were isolated and sorted into CD44+CD24+ ,CD44-CD24+ ,CD44+CD24- ,and CD44-CD24- .Multi‐target click model was used to fit cell survival curves and deter‐mine the sensitizer enhancement ratio .The apoptosis and cycle distribution of the four cell subsets were determined using flow cy‐tometry ,and the level of ROS was determined by DCFH‐DA probe .Results The ratio of CD44+ and CD24+ in the sorted PANC‐1 cell line was 92 .0% and 4 .7% respectively .Before radiation ,there was no statistically significant difference between four groups (P>0 .05);After treated with 6MV‐X ray ,The ratio of apoptosis was the lowest in CD44+CD24+ (P<0 .01);The percentage G0/G1 cell was the highest in CD44+CD24+ (P<0 .01) ,the sensitizer enhancement ratio of CD44+ CD24- ,CD44-CD24+ and CD44-CD24- were 1 .61 ,1 .81 ,1 .94 ,respectively .The level of ROS in CD44+CD24+ was lower (P<0 .01) .Conclusion Tumor stem cells of pancreatic adenocarcinoma have properties of a lower level of ROS and relative stationary that maybe the reasons of radio resist‐ance .
