重庆医学
重慶醫學
중경의학
Chongqing Medicine
2015年
32期
4490-4492
,共3页
周雅菲%朱显婷%邓雨晴%周威
週雅菲%硃顯婷%鄧雨晴%週威
주아비%주현정%산우청%주위
石菖蒲%AB-8大孔树脂不同洗脱部位%高效液相图谱%抗抑郁
石菖蒲%AB-8大孔樹脂不同洗脫部位%高效液相圖譜%抗抑鬱
석창포%AB-8대공수지불동세탈부위%고효액상도보%항억욱
acorus tatarinowii schott%different fraction eluted from AB-8 macroporous resin%high performance liquid chro-matography%anti-depression
目的：研究石菖蒲大孔树脂不同洗脱部位高效液相图谱与抗抑郁效应的关联性。方法采用AB‐8大孔树脂制备石菖蒲不同部位。采用梯度洗脱法建立石菖蒲原药材及不同树脂洗脱部位的高效液相特征图谱，并确认图谱中特征峰及对应特征峰之间的峰面积数据关系。运用小鼠悬尾实验（TST）、游泳实验（FST）等小鼠行为学实验，观察口服石菖蒲及不同树脂洗脱部位抗抑郁效应。结果石菖蒲原药材及不同AB‐8树脂洗脱部位的特征图谱具有特征共有峰。石菖蒲大孔树脂90％及70％乙醇洗脱部分能显著缩短TST和FST。石菖蒲抗抑郁效应与特征峰具有关联性。结论石菖蒲具有抗抑郁作用，其主要抗抑郁效应来源于AB‐8树脂70％及90％乙醇洗脱部位。石菖蒲液相特征图谱及相关峰数据关系可用于衡量石菖蒲的抗抑郁效应，为今后石菖蒲用于抗抑郁效应研究及开发提供依据。
目的：研究石菖蒲大孔樹脂不同洗脫部位高效液相圖譜與抗抑鬱效應的關聯性。方法採用AB‐8大孔樹脂製備石菖蒲不同部位。採用梯度洗脫法建立石菖蒲原藥材及不同樹脂洗脫部位的高效液相特徵圖譜，併確認圖譜中特徵峰及對應特徵峰之間的峰麵積數據關繫。運用小鼠懸尾實驗（TST）、遊泳實驗（FST）等小鼠行為學實驗，觀察口服石菖蒲及不同樹脂洗脫部位抗抑鬱效應。結果石菖蒲原藥材及不同AB‐8樹脂洗脫部位的特徵圖譜具有特徵共有峰。石菖蒲大孔樹脂90％及70％乙醇洗脫部分能顯著縮短TST和FST。石菖蒲抗抑鬱效應與特徵峰具有關聯性。結論石菖蒲具有抗抑鬱作用，其主要抗抑鬱效應來源于AB‐8樹脂70％及90％乙醇洗脫部位。石菖蒲液相特徵圖譜及相關峰數據關繫可用于衡量石菖蒲的抗抑鬱效應，為今後石菖蒲用于抗抑鬱效應研究及開髮提供依據。
목적：연구석창포대공수지불동세탈부위고효액상도보여항억욱효응적관련성。방법채용AB‐8대공수지제비석창포불동부위。채용제도세탈법건립석창포원약재급불동수지세탈부위적고효액상특정도보，병학인도보중특정봉급대응특정봉지간적봉면적수거관계。운용소서현미실험（TST）、유영실험（FST）등소서행위학실험，관찰구복석창포급불동수지세탈부위항억욱효응。결과석창포원약재급불동AB‐8수지세탈부위적특정도보구유특정공유봉。석창포대공수지90％급70％을순세탈부분능현저축단TST화FST。석창포항억욱효응여특정봉구유관련성。결론석창포구유항억욱작용，기주요항억욱효응래원우AB‐8수지70％급90％을순세탈부위。석창포액상특정도보급상관봉수거관계가용우형량석창포적항억욱효응，위금후석창포용우항억욱효응연구급개발제공의거。
Objective To research the correlation on high performance liquid chromatography (HPLC) and antidepressant effect of different elution fractions of acorus tatarinowii schott macroporous resin .Methods Prepared different fractions by using AB‐8 macroporous resin .Establish HPLC characteristic chromatograms of crude drugs and different elution fractions from AB‐8 acorus tatarinowii schott macroporous resin by using gradient elusion .Confirm characteristic peaks in chromatograms .despair mod‐els of male mice ,including tails suspension test (TST ) ,forced swimming test (FST ) were used to evaluate antidepressant effects by taking orally .Results HPLC chromatograms of crude drugs and different fraction of acorus tatarinowii schott showed that they had same characteristic peaks .Elution of 90% and 70% alcohol solution from AB‐8 macroporous resin can obviously reduce TST and FST time .The anti‐depressant effect were associated with characteristic peaks on chromatogram .Conclusion Acorus tatarinowii schott has anti‐depressant effect ,and mainly from 70% and 90% alcohol solution eluted from AB‐8 macroporous resin .HPLC char‐acteristic chromatography and data relationship between relative peaks can be used to judge the antidepressant effects of acorus ta‐tarinowii schott ,and give reference to research and product development .
