CAJ | 학술논문

目的：探讨室壁瘤形成过程中基质金属蛋白酶‐9（MMP‐9）血清浓度与心功能及室壁瘤容积的相关性。方法20只新西兰大白兔结扎左前降支及左回旋支制作室壁瘤模型。于术前，术后1、2、3d，术后1、2、3、4周进行常规超声心动图检查，同时耳缘静脉抽血，至术后4周有室壁瘤形成的实验兔纳入研究。使用实时三维超声心动图测量左心室容积、左心室射血分数（LVEF）及室壁瘤容积（LVAV）。采用ELISA法进行MMP‐9血清浓度测定。结果（1）术后1 d MMP‐9血清浓度开始增高，术后3 d达到高峰，至术后4周仍然高于术前，差异有统计学意义（P＜0．05）。（2）与术前比较，术后各时间段的左心室舒张末期容积（LVEDV）、左心室收缩末期容积（LVESV）、LVAV均增大（P＜0．05），LVEF下降（P＜0．05）。（3）直线相关性显示：术后各时间段MMP‐9血清浓度与LVEF均具有相关性，其中术后3 d MMP‐9血清浓度与LVEF相关性较好（r＝0．731，P＜0．05）。术后2 d后各时间段MMP‐9血清浓度与LVAV均具有相关性，其中术后3 d MMP‐9血清浓度与LVAV相关性较好（r＝0．636，P＜0．05）。结论 MMP‐9在室壁瘤形成过程中对心功能和LVAV有一定的影响作用。
목적：탐토실벽류형성과정중기질금속단백매‐9（MMP‐9）혈청농도여심공능급실벽류용적적상관성。방법20지신서란대백토결찰좌전강지급좌회선지제작실벽류모형。우술전，술후1、2、3d，술후1、2、3、4주진행상규초성심동도검사，동시이연정맥추혈，지술후4주유실벽류형성적실험토납입연구。사용실시삼유초성심동도측량좌심실용적、좌심실사혈분수（LVEF）급실벽류용적（LVAV）。채용ELISA법진행MMP‐9혈청농도측정。결과（1）술후1 d MMP‐9혈청농도개시증고，술후3 d체도고봉，지술후4주잉연고우술전，차이유통계학의의（P＜0．05）。（2）여술전비교，술후각시간단적좌심실서장말기용적（LVEDV）、좌심실수축말기용적（LVESV）、LVAV균증대（P＜0．05），LVEF하강（P＜0．05）。（3）직선상관성현시：술후각시간단MMP‐9혈청농도여LVEF균구유상관성，기중술후3 d MMP‐9혈청농도여LVEF상관성교호（r＝0．731，P＜0．05）。술후2 d후각시간단MMP‐9혈청농도여LVAV균구유상관성，기중술후3 d MMP‐9혈청농도여LVAV상관성교호（r＝0．636，P＜0．05）。결론 MMP‐9재실벽류형성과정중대심공능화LVAV유일정적영향작용。
Objective To explore the correlation between matrix metalloproteinase‐9(MMP‐9) and cardiac function ,aneu‐rysm volume in the process of left ventricular aneurysm (LVA) formation .Methods Rabbit models of LVA were established in 20 New Zealand rabbits by lighting left anterior descending artery and left circumflex artery .Two‐dimensional echocardiography were performed at preoperative and postoperative 1 d ,2 d ,3 d ,1 w ,2 w ,3 w ,4 w .The survived animals which had LVA formed were en‐rolled .Real time three‐dimensional echocardiography was performed to obtain left ventricular volume ,LVEF and LVA volume ,The MMP‐9 serum concentration was measured by ELISA .Results (1)There were a significant increase in the serum concentration of MMP‐9 from postoperative 1 d and arrived at the peak at postoperative 3 d ,there were higher than preoperative at postoperative 4 w (P<0 .05) .(2)Compared with preoperative ,the LVEDV ,LVESV and LVAV volume at postoperative showed an increase trend (P<0 .05) ,while there was a decreasing trend in LVEF(P<0 .05) .(3)The MMP‐9 had the better relationship with LVEF at post‐operative 1 d ,2 d ,3 d ,1 w ,2 w ,3 w ,4 w ,in which MMP‐9 had the tightest relationship with LVEF at postoperative 3 d (r=0 .731 , P<0 .05) .MMP‐9 had the better relationship with LVA volume at postoperative 2 d ,3 d ,1 w ,2 w ,3 w ,4 w ,in which MMP‐9 had the tightest relationship with LVA volume at postoperative 3 d (r=0 .636 ,P<0 .05) .Conclusion The MMP‐9 had an effect on cardiac function and LVA volume in the process of LVA formation .