CAJ | 학술논문

目的：检测人胎胃成纤维细胞人端粒酶逆转录酶（hT ERT ）和端粒酶表达。方法分离培养人胎胃成纤维细胞，细胞角蛋白（CK）‐18免疫染色排除上皮细胞；免疫细胞化学方法检测hTERT表达；端粒重复序列扩增法（TRAP）检测端粒酶活性，成人胃成纤维细胞作阳性对照。结果分离的人胎胃成纤维细胞CK‐18免疫染色阴性；原位监测其胞质和胞核中均可见细颗粒状免疫荧光；端粒酶延伸片断长度约为170 bp ，稍长于成人胃成纤维细胞。结论人胎胃成纤维细胞表达hT ERT和端粒酶。
목적：검측인태위성섬유세포인단립매역전록매（hT ERT ）화단립매표체。방법분리배양인태위성섬유세포，세포각단백（CK）‐18면역염색배제상피세포；면역세포화학방법검측hTERT표체；단립중복서렬확증법（TRAP）검측단립매활성，성인위성섬유세포작양성대조。결과분리적인태위성섬유세포CK‐18면역염색음성；원위감측기포질화포핵중균가견세과립상면역형광；단립매연신편단장도약위170 bp ，초장우성인위성섬유세포。결론인태위성섬유세포표체hT ERT화단립매。
Objective To detect the expression of human telomerase reverse transcriptase (hTERT ) and telomerase in hu‐man fetal gastric fibroblasts .Methods Human fetal gastric fibroblasts were isolated and cultured ,epithelial cells were exduded by CK‐18 immune staining .hTERT protein was determined by indirect immunofluorescence .Telomerase activity was analyzed using telomeric repeat amplification protocol assay (TRAP)while the same analysis in adult gastric fibroblast ,which as to be positive con‐trol .Results Cultured fibroblasts were CK‐18 negative .The positive hTERT immunostaining was detected in both cellular cyto‐plasm and nuclear compartments .Amplified telomeric repeats (about 170 bp) in human fetal gastric fibroblasts were longer than those (160 bp) in adult gastric fibroblasts .Conclusion hTERT and telomerase were expressed in human fetal gastric fibroblasts .