中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
Chinese Journal of Plastic Surgery
2015年
6期
446-450
,共5页
陈淑明%陈少全%张再重%黎成金%夏印%林晨%黄庆锦%王烈
陳淑明%陳少全%張再重%黎成金%夏印%林晨%黃慶錦%王烈
진숙명%진소전%장재중%려성금%하인%림신%황경금%왕렬
普萘洛尔%水凝胶%血管瘤%模型,动物
普萘洛爾%水凝膠%血管瘤%模型,動物
보내락이%수응효%혈관류%모형,동물
Propranolol%Hydrochloride gel%Hemangioma%Models,animal
目的 探讨普萘洛尔凝胶治疗裸鼠血管瘤的有效性及安全性.方法 BALB /c(nu/nu)裸鼠30只,通过简单随机化分组法分为实验组、对照组和正常组,每组10只,于实验组和对照组裸鼠皮下注射血管瘤内皮细胞建立血管瘤模型,以3％普萘洛尔凝胶外涂实验组注射部位及其周围约0.5 cm正常皮肤,以及正常组裸鼠皮肤,对照组等待观察.定期记录、观察瘤体的生长情况,记录涂药部位皮肤水肿、红斑评分情况.细胞注射45 d后,处死裸鼠,以高效液相色谱荧光检测法检测实验组和正常组裸鼠普萘洛尔血药浓度,HE染色及免疫组化鉴定对照组瘤体组织CD31、CD34抗原表达情况.组间差异比较采用x2检验,计量资料采用均数±标准差表示.采用配对样本资料的t检验分析普萘洛尔凝胶外涂后瘤体体积、血药浓度及裸鼠皮肤变化情况.结果 细胞注射45 d,对照组瘤体体积为(366.57±17.08) mm3,明显高于实验组的(13.36±2.09) mm3,2组比较差异有统计学意义(P＜0.05);正常组血药浓度为(16.83±1.53) ng/ml,实验组为(18.42 ±2.21) ng/ml,2组比较差异无统计学意义(P＞0.05).实验组与对照组皮肤刺激分值均为0分.结论 局部外涂普萘洛尔凝胶可以抑制裸鼠血管瘤瘤体的生长,皮肤刺激性小,可以长期安全使用.
目的 探討普萘洛爾凝膠治療裸鼠血管瘤的有效性及安全性.方法 BALB /c(nu/nu)裸鼠30隻,通過簡單隨機化分組法分為實驗組、對照組和正常組,每組10隻,于實驗組和對照組裸鼠皮下註射血管瘤內皮細胞建立血管瘤模型,以3％普萘洛爾凝膠外塗實驗組註射部位及其週圍約0.5 cm正常皮膚,以及正常組裸鼠皮膚,對照組等待觀察.定期記錄、觀察瘤體的生長情況,記錄塗藥部位皮膚水腫、紅斑評分情況.細胞註射45 d後,處死裸鼠,以高效液相色譜熒光檢測法檢測實驗組和正常組裸鼠普萘洛爾血藥濃度,HE染色及免疫組化鑒定對照組瘤體組織CD31、CD34抗原錶達情況.組間差異比較採用x2檢驗,計量資料採用均數±標準差錶示.採用配對樣本資料的t檢驗分析普萘洛爾凝膠外塗後瘤體體積、血藥濃度及裸鼠皮膚變化情況.結果 細胞註射45 d,對照組瘤體體積為(366.57±17.08) mm3,明顯高于實驗組的(13.36±2.09) mm3,2組比較差異有統計學意義(P＜0.05);正常組血藥濃度為(16.83±1.53) ng/ml,實驗組為(18.42 ±2.21) ng/ml,2組比較差異無統計學意義(P＞0.05).實驗組與對照組皮膚刺激分值均為0分.結論 跼部外塗普萘洛爾凝膠可以抑製裸鼠血管瘤瘤體的生長,皮膚刺激性小,可以長期安全使用.
목적 탐토보내락이응효치료라서혈관류적유효성급안전성.방법 BALB /c(nu/nu)라서30지,통과간단수궤화분조법분위실험조、대조조화정상조,매조10지,우실험조화대조조라서피하주사혈관류내피세포건립혈관류모형,이3％보내락이응효외도실험조주사부위급기주위약0.5 cm정상피부,이급정상조라서피부,대조조등대관찰.정기기록、관찰류체적생장정황,기록도약부위피부수종、홍반평분정황.세포주사45 d후,처사라서,이고효액상색보형광검측법검측실험조화정상조라서보내락이혈약농도,HE염색급면역조화감정대조조류체조직CD31、CD34항원표체정황.조간차이비교채용x2검험,계량자료채용균수±표준차표시.채용배대양본자료적t검험분석보내락이응효외도후류체체적、혈약농도급라서피부변화정황.결과 세포주사45 d,대조조류체체적위(366.57±17.08) mm3,명현고우실험조적(13.36±2.09) mm3,2조비교차이유통계학의의(P＜0.05);정상조혈약농도위(16.83±1.53) ng/ml,실험조위(18.42 ±2.21) ng/ml,2조비교차이무통계학의의(P＞0.05).실험조여대조조피부자격분치균위0분.결론 국부외도보내락이응효가이억제라서혈관류류체적생장,피부자격성소,가이장기안전사용.
Objective To explore the effect and safety of topical propranolol hydrochloride gel for treatment of infantile hemangioma.Mehods Thirty nude mice(BALA/c, nu/nu)were divided into three groups, experimental group, control group and normal group.Human hemangioma endothelial cells cultured in vitro were injected subcutaneously in experimental group and control group to establish infantile hemangioma model.Topical propranolol hydrochloride gel was applied on the surface of the hemangioman in experimental group and normal group.Tumor volumn change and the skin situations (edema, erythema, ulceration) were observed at different periods.45 days after cell injection, the mice were killed and plasma concentration was detected in the experimental group and the control group by high performance liquid chromatography with evaporative light scattering detector, and tumors were subjected to histopathologic examination and immunohistochemistry for CD31 and CD34.The correlation between volumes and plasma concentration was statistically analyzed with SPSS 13.0 paired samples t test with α =0.05 as statistical standard.Results At 45 days, the volume of the tumor in control group was (366.57 ± 17.08) mm3 ,which has a significant difference as compared to the experimental group(13.36 ± 2.09) mm3(P ＜ 0.05);and the plasma concentration was (16.83 ± 1.53) ng/ml in experimental group, and (18.42 ± 2.21) ng/ ml in normal group (P ＞ 0.05).Topical propranolol hydrochloride gel (3％) has no irritation to nude mice's skin.Conclusions Topical application of 3％ propranolol hydrochloride gel is effective and safe for the treatment of infantile hemangioma.
