中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2015年
11期
819-824
,共6页
胡超%钱丹琪%秦颂兵%叶超%周菊英
鬍超%錢丹琪%秦頌兵%葉超%週菊英
호초%전단기%진송병%협초%주국영
放射增敏%低温等离子体%HepG2细胞%HeLa细胞%A549细胞
放射增敏%低溫等離子體%HepG2細胞%HeLa細胞%A549細胞
방사증민%저온등리자체%HepG2세포%HeLa세포%A549세포
Radiosensitization%Low-temperature plasma%HepG2 cells%HeLa cells%A549 cells
目的 探讨低温等离子体对人肝癌细胞系HepG2、非小细胞肺癌细胞系A549及人宫颈癌细胞系HeLa的放射增敏作用及其机制.方法 应用克隆形成实验观察低温等离子体对3种细胞的放射增敏作用;流式细胞仪分析3种细胞周期分布、凋亡率及活性氧含量;Western blot法检测单纯照射(R组)、单纯等离子体作用(P组)及其联合辐射(PsR组)对3种细胞中Bcl-2、Caspase-3 蛋白表达的影响.结果 低温等离子体对HepG2、A549及HeLa细胞均有放射增敏作用,放射增敏比(SERD0)分别为1.28、1.32、1.29.HepG2、A549及HeLa细胞P+R组G2/M期比例、凋亡率及活性氧含量与R组比较均明显增高(tG2/M期=9.52、8.24、9.53,P＜0.05;t凋亡率=10.67、38.56、6.74,P＜0.05;t活性氧含量=9.41、15.42、13.53,P＜0.05).HepG2和A549细胞P+R组C2/M期比例、凋亡率及活性氧含量与P组比较均明显升高(tG2/M期=8.75、20.37,P＜0.05;t凋亡率 =8.43、9.99,P＜0.05;t活性氧含量=4.82、5.27,P＜0.05).3种癌细胞中P+R组Bcl-2蛋白表达较R组降低,而Caspase-3蛋白表达升高.结论 低温等离子体可提高HepG2、A549及HeLa细胞系的放射敏感性,其对HepG2及A549细胞系的放射增敏机制可能与抑制亚致死损伤修复,使细胞周期阻滞在G2/M期,以及提高细胞内ROS水平诱导细胞凋亡有关.
目的 探討低溫等離子體對人肝癌細胞繫HepG2、非小細胞肺癌細胞繫A549及人宮頸癌細胞繫HeLa的放射增敏作用及其機製.方法 應用剋隆形成實驗觀察低溫等離子體對3種細胞的放射增敏作用;流式細胞儀分析3種細胞週期分佈、凋亡率及活性氧含量;Western blot法檢測單純照射(R組)、單純等離子體作用(P組)及其聯閤輻射(PsR組)對3種細胞中Bcl-2、Caspase-3 蛋白錶達的影響.結果 低溫等離子體對HepG2、A549及HeLa細胞均有放射增敏作用,放射增敏比(SERD0)分彆為1.28、1.32、1.29.HepG2、A549及HeLa細胞P+R組G2/M期比例、凋亡率及活性氧含量與R組比較均明顯增高(tG2/M期=9.52、8.24、9.53,P＜0.05;t凋亡率=10.67、38.56、6.74,P＜0.05;t活性氧含量=9.41、15.42、13.53,P＜0.05).HepG2和A549細胞P+R組C2/M期比例、凋亡率及活性氧含量與P組比較均明顯升高(tG2/M期=8.75、20.37,P＜0.05;t凋亡率 =8.43、9.99,P＜0.05;t活性氧含量=4.82、5.27,P＜0.05).3種癌細胞中P+R組Bcl-2蛋白錶達較R組降低,而Caspase-3蛋白錶達升高.結論 低溫等離子體可提高HepG2、A549及HeLa細胞繫的放射敏感性,其對HepG2及A549細胞繫的放射增敏機製可能與抑製亞緻死損傷脩複,使細胞週期阻滯在G2/M期,以及提高細胞內ROS水平誘導細胞凋亡有關.
목적 탐토저온등리자체대인간암세포계HepG2、비소세포폐암세포계A549급인궁경암세포계HeLa적방사증민작용급기궤제.방법 응용극륭형성실험관찰저온등리자체대3충세포적방사증민작용;류식세포의분석3충세포주기분포、조망솔급활성양함량;Western blot법검측단순조사(R조)、단순등리자체작용(P조)급기연합복사(PsR조)대3충세포중Bcl-2、Caspase-3 단백표체적영향.결과 저온등리자체대HepG2、A549급HeLa세포균유방사증민작용,방사증민비(SERD0)분별위1.28、1.32、1.29.HepG2、A549급HeLa세포P+R조G2/M기비례、조망솔급활성양함량여R조비교균명현증고(tG2/M기=9.52、8.24、9.53,P＜0.05;t조망솔=10.67、38.56、6.74,P＜0.05;t활성양함량=9.41、15.42、13.53,P＜0.05).HepG2화A549세포P+R조C2/M기비례、조망솔급활성양함량여P조비교균명현승고(tG2/M기=8.75、20.37,P＜0.05;t조망솔 =8.43、9.99,P＜0.05;t활성양함량=4.82、5.27,P＜0.05).3충암세포중P+R조Bcl-2단백표체교R조강저,이Caspase-3단백표체승고.결론 저온등리자체가제고HepG2、A549급HeLa세포계적방사민감성,기대HepG2급A549세포계적방사증민궤제가능여억제아치사손상수복,사세포주기조체재G2/M기,이급제고세포내ROS수평유도세포조망유관.
Objective To evaluate the radiosensitization effect of low-temperature plasma on HepG2, A549, and HeLa cells.Methods Cells were divided into three groups, radiation group (R) , plasma treatment group(P), and plasma plus radiation group (P + R).After radiation, cell survival was detected by a cloning assay.Cell cycle distribution, apoptosis and ROS content were tested by flow cytometry.Western blot was used to measure the expressions of Caspase-3 and Bcl-2.Results Lowtemperature plasma showed radiosensitization effects on three different human malignant cell lines with a sensitivity enhancement ratio(SERD0) of 1.28,1.32 and 1.29.respectively.In these three different human malignant cell lines, compared with radiation alone group (R) , the G2/M arrest, apoptosis rate and ROS level in the group P + R were enhanced (the prolongation of G2/M arrest: t =9.52, 8.24, 9.53, P ＜ 0.05;the apoptosis rate: t =10.67, 38.56, 6.74, P ＜0.05;ROS content: t =9.41, 15.42, 13.53, P ＜0.05).In HepG2 cells and A549 cells, compared with group P, the prolongation of G2/M arrest, the apoptosis rate and ROS content of group P + R were enhanced (the prolongation of G2/M arrest: t =8.75, 20.37, P＜0.05;the apoptosis rate: t =8.43, 9.99, P ＜0.05;ROS content: t =4.82, 5.27, P ＜ 0.05).The expression level of Bcl-2 protein was downregulated in group P + R;by contrast, the expression level of Caspase-3 protein in group P + R was upregulated.Conclusions Low-temperature plasma can increase the radiosensitization of HepG2, A549 and HeLa cells with the enhancement of G2/M phase arrest, apoptosis induction and ROS generation.
