CAJ | 학술논문

目的：采用 MALDI‐TOF 质谱分析方法鉴定院内感染致病菌。方法同步使用 VITEK‐2和 MALDI‐TOF 质谱鉴定院内感染致病菌。从检测时间、鉴定率及检出数量进行两种方法的对比。将微生物大分子指纹图谱进行共性峰比对计算，获得院内感染致病菌株间同源性结构关系的差异。结果共检出31株大肠埃希氏菌、28株肺炎克雷伯氏菌及解鸟苷酸拉乌尔菌等罕见致病菌9株。 MALDI‐TOF 质谱检测能够在1 h 内检出所有的致病菌，且鉴定率和检出数量高于 VITEK‐2。结论致病菌的院内感染呈现科室为中心点源传播的模式。 MALDI‐TOF 质谱检测速度快、准确率高，能够进行临床耐药菌的聚类分析，有望成为致病菌鉴定和临床耐药菌风险监测工作的主要技术力量。
목적：채용 MALDI‐TOF 질보분석방법감정원내감염치병균。방법동보사용 VITEK‐2화 MALDI‐TOF 질보감정원내감염치병균。종검측시간、감정솔급검출수량진행량충방법적대비。장미생물대분자지문도보진행공성봉비대계산，획득원내감염치병균주간동원성결구관계적차이。결과공검출31주대장애희씨균、28주폐염극뢰백씨균급해조감산랍오이균등한견치병균9주。 MALDI‐TOF 질보검측능구재1 h 내검출소유적치병균，차감정솔화검출수량고우 VITEK‐2。결론치병균적원내감염정현과실위중심점원전파적모식。 MALDI‐TOF 질보검측속도쾌、준학솔고，능구진행림상내약균적취류분석，유망성위치병균감정화림상내약균풍험감측공작적주요기술역량。
Objective Pathogens from the nosocomial infection have been analyzed by MALDI‐TOF microbial identification system ,to evaluate mass spectrometry analysis advantage and explore the mass spectrometry method .Methods The pathogens have been analyzed by MALDI‐TOF microbial identification system ,by compared with the VITEK‐2 compact detection in the tes‐ting time ,detection rate and the amounts of identified strains .The homology differences have been analyzed by comparison calcula‐tion of common peaks from the fingerprint spectrums .Results Thirty‐one Escherichia coli strains ,28 Klebsiella pneumonia strains and 9 unusual pathogen strains have been identified by MALDI‐TOF MS for only 1 hours .It has more advantages than VITEK‐2 in the testing time and other aspects .Conclusion Nosocomial infection of pathogen shows a point source propagation mode centering on the department .MALDI‐TOF mass spectrometry is able to rapidly and correctly identify the pathogen .MALDI‐TOF microbial i‐dentification system is expected to be the major detecting technique in the field of the pathogen monitor and resistance monitoring a ‐nalysis .