重庆医学
重慶醫學
중경의학
Chongqing Medicine
2015年
33期
4611-4614
,共4页
丹酚酸 B%血脑屏障%缺氧
丹酚痠 B%血腦屏障%缺氧
단분산 B%혈뇌병장%결양
salvianolic acid B%blood brain-barrier%anoxia
目的:探讨丹酚酸 B 对体外血脑屏障缺糖缺氧/复糖复氧的保护作用。方法利用人脑微血管内皮细胞与 C6细胞非接触共培养建立体外血脑屏障模型并分为正常组、实验组和给药组。利用无糖 Earle′s 液代替培养液在93% N2、2% O2和5% CO2条件下孵育2 h 模拟缺糖缺氧环境。辣根过氧化物酶通透性实验检测血脑屏障通透性变化。免疫荧光和电子显微镜观察紧密连接变化情况。结果给药组对辣根过氧化物酶的通透性明显低于实验组(P <0.05);免疫荧光和超微结构图片显示正常的紧密连接连续和致密,而实验组紧密连结中断和疏松,给药组介于二者之间。结论丹酚酸 B 能直接保护缺糖缺氧/复糖复氧引起的血脑屏破坏和降低通透性,为丹酚酸 B 对血脑屏障的保护机制提供的研究基础。
目的:探討丹酚痠 B 對體外血腦屏障缺糖缺氧/複糖複氧的保護作用。方法利用人腦微血管內皮細胞與 C6細胞非接觸共培養建立體外血腦屏障模型併分為正常組、實驗組和給藥組。利用無糖 Earle′s 液代替培養液在93% N2、2% O2和5% CO2條件下孵育2 h 模擬缺糖缺氧環境。辣根過氧化物酶通透性實驗檢測血腦屏障通透性變化。免疫熒光和電子顯微鏡觀察緊密連接變化情況。結果給藥組對辣根過氧化物酶的通透性明顯低于實驗組(P <0.05);免疫熒光和超微結構圖片顯示正常的緊密連接連續和緻密,而實驗組緊密連結中斷和疏鬆,給藥組介于二者之間。結論丹酚痠 B 能直接保護缺糖缺氧/複糖複氧引起的血腦屏破壞和降低通透性,為丹酚痠 B 對血腦屏障的保護機製提供的研究基礎。
목적:탐토단분산 B 대체외혈뇌병장결당결양/복당복양적보호작용。방법이용인뇌미혈관내피세포여 C6세포비접촉공배양건입체외혈뇌병장모형병분위정상조、실험조화급약조。이용무당 Earle′s 액대체배양액재93% N2、2% O2화5% CO2조건하부육2 h 모의결당결양배경。랄근과양화물매통투성실험검측혈뇌병장통투성변화。면역형광화전자현미경관찰긴밀련접변화정황。결과급약조대랄근과양화물매적통투성명현저우실험조(P <0.05);면역형광화초미결구도편현시정상적긴밀련접련속화치밀,이실험조긴밀련결중단화소송,급약조개우이자지간。결론단분산 B 능직접보호결당결양/복당복양인기적혈뇌병파배화강저통투성,위단분산 B 대혈뇌병장적보호궤제제공적연구기출。
Objective To explore the influence of salvianolic acid B(SalB) to blood brain‐barrier(BBB) in vitro oxygen/glu‐cose deprivation and reintroduction .Methods We used the human brain microvascular endothelial cells and C6 cells to build BBB model that in vitro ,and the models was divided into normal groups ,experiment groups ,treatment groups .The Earle′s liquid and the condition of 93% N2 ,2% O2 ,5% CO2 replaced the normal culture environment to simulate environment of oxygen /glucose depriva‐tion and reintroduction .The changes of BBB permeability was detected by the horseradish peroxidase permeability test .The tight junctions(TJs) was detected by immunofluorescence and electron microscope .Results The permeability of treatment group was significantly lower than the experiment group (P < 0 .05) ;The TJs of normal group was continuous and density ;the experiment group′s TJs was interruption and osteoporosis ;and the observation of treatment group′s TJs was between of the two group above . Conclusion The SalB can directly protect the BBB during oxygen /glucose deprivation and reintroduction ,and decrease the permea‐bility .The investigation also provided the research foundation for the SalB to protect the BBB .