中国基层医药
中國基層醫藥
중국기층의약
Chinese Journal of Primary Medicine and Pharmacy
2015年
23期
3564-3565,3566
,共3页
巧克力培养基%羊血%流感嗜血杆菌%检验技术与方法
巧剋力培養基%羊血%流感嗜血桿菌%檢驗技術與方法
교극력배양기%양혈%류감기혈간균%검험기술여방법
Chocolate medium%Sheep blood%Haemophilus influenzae%Testing techniques and methods
目的：评价改良羊血巧克力培养基的质量与应用价值。方法将 ATCC 10211流感嗜血杆菌接种到改良前后两种培养基上，比较两种培养基上嗜血杆菌的平均生长指数（GI 值）；对352份经筛选的合格痰标本进行流感嗜血杆菌检测，比较两种培养基的阳性分离率。结果传统羊血巧克力培养基 GI 值为（3．69±0．58），改良羊血巧克力培养基 GI 值为（15．08±1．34），两种培养基 GI 值差异有统计学意义（t ＝25．31，P ＜0.01）。352份痰标本在改良羊血巧克力培养基上流感嗜血杆菌检出41例，阳性检出率11．65％。352份痰标本在传统羊血巧克力培养基上流感嗜血杆菌检出18例，阳性检出率5．54％。流感嗜血杆菌在两种巧克力培养基上的分离率差异有统计学意义（χ2＝21．04，P ＜0．05）。结论流感嗜血杆菌在改良羊血巧克力培养基上生长良好，菌落明显，极易识别，有助于临床标本中流感嗜血杆菌的检出。
目的：評價改良羊血巧剋力培養基的質量與應用價值。方法將 ATCC 10211流感嗜血桿菌接種到改良前後兩種培養基上，比較兩種培養基上嗜血桿菌的平均生長指數（GI 值）；對352份經篩選的閤格痰標本進行流感嗜血桿菌檢測，比較兩種培養基的暘性分離率。結果傳統羊血巧剋力培養基 GI 值為（3．69±0．58），改良羊血巧剋力培養基 GI 值為（15．08±1．34），兩種培養基 GI 值差異有統計學意義（t ＝25．31，P ＜0.01）。352份痰標本在改良羊血巧剋力培養基上流感嗜血桿菌檢齣41例，暘性檢齣率11．65％。352份痰標本在傳統羊血巧剋力培養基上流感嗜血桿菌檢齣18例，暘性檢齣率5．54％。流感嗜血桿菌在兩種巧剋力培養基上的分離率差異有統計學意義（χ2＝21．04，P ＜0．05）。結論流感嗜血桿菌在改良羊血巧剋力培養基上生長良好，菌落明顯，極易識彆，有助于臨床標本中流感嗜血桿菌的檢齣。
목적：평개개량양혈교극력배양기적질량여응용개치。방법장 ATCC 10211류감기혈간균접충도개량전후량충배양기상，비교량충배양기상기혈간균적평균생장지수（GI 치）；대352빈경사선적합격담표본진행류감기혈간균검측，비교량충배양기적양성분리솔。결과전통양혈교극력배양기 GI 치위（3．69±0．58），개량양혈교극력배양기 GI 치위（15．08±1．34），량충배양기 GI 치차이유통계학의의（t ＝25．31，P ＜0.01）。352빈담표본재개량양혈교극력배양기상류감기혈간균검출41례，양성검출솔11．65％。352빈담표본재전통양혈교극력배양기상류감기혈간균검출18례，양성검출솔5．54％。류감기혈간균재량충교극력배양기상적분리솔차이유통계학의의（χ2＝21．04，P ＜0．05）。결론류감기혈간균재개량양혈교극력배양기상생장량호，균락명현，겁역식별，유조우림상표본중류감기혈간균적검출。
Objective To evaluate the quality and application value of improved sheep blood chocolate medium.Methods The ATCC 10211 of Haemophilus influenzae was inoculated into the modified medium and unmodified medium,the average growth index(GI)of Haemophilus influenzae in two kinds of culture medium was compared.Based on 352 selected qualified sputum specimens for detection of Haemophilus influenzae,the positive isolation rate of medium was compared between the two groups.Results GI value of the traditional blood chocolate culture medium was (3.69 ±0.58),which was significantly lower than (15.08 ±1.34)of the improved sheep blood chocolate culture medium,the difference was significant (t =25.31,P <0.01 ).352 sputum specimens in the improved sheep blood chocolate culture medium,Haemophilus influenzae detected in 41 cases,the positive rate was 11.65%.And 352 sputum specimens in traditional sheep blood chocolate culture,Haemophilus influenzae detected in 18 cases,the positive rate was 5.54%.There was significant differenceof Haemophilus influenzae in separation based on 2 kinds of chocolate culture medium (χ2 =21.04,P <0.05).Conclusion Haemophilus influenzae significantly improved sheep blood colony in chocolate culture medium,grows well,easily to be identified,which helps to detect sputum specimens of Haemophilus influenzae.
