CAJ | 학술논문

目的：观察14-3-3蛋白在哮喘大鼠肺组织中的表达，探讨其参与哮喘炎性反应的机制。方法采用免疫组化方法检测大鼠肺组织14-3-3蛋白的表达水平，分析其与哮喘炎性反应的关系。结果14-3-3蛋白主要在细胞浆中表达，部分表达于细胞膜，阳性细胞主要有气道上皮细胞，其次为淋巴细胞、肺泡巨噬细胞和血管内皮细胞，支气管平滑肌和血管平滑肌均呈阴性表达。哮喘组肺组织14-3-3蛋白的表达强度[（0．353±0．023）A 值]显著高于对照组[（0．211±0．028）A 值]（t ＝10．969，P ＜0．01）。结论哮喘大鼠肺组织14-3-3蛋白表达增强，它可能主要通过支气管上皮细胞、淋巴细胞、肺泡巨噬细胞和血管内皮细胞参与哮喘的炎症机制。
목적：관찰14-3-3단백재효천대서폐조직중적표체，탐토기삼여효천염성반응적궤제。방법채용면역조화방법검측대서폐조직14-3-3단백적표체수평，분석기여효천염성반응적관계。결과14-3-3단백주요재세포장중표체，부분표체우세포막，양성세포주요유기도상피세포，기차위림파세포、폐포거서세포화혈관내피세포，지기관평활기화혈관평활기균정음성표체。효천조폐조직14-3-3단백적표체강도[（0．353±0．023）A 치]현저고우대조조[（0．211±0．028）A 치]（t ＝10．969，P ＜0．01）。결론효천대서폐조직14-3-3단백표체증강，타가능주요통과지기관상피세포、림파세포、폐포거서세포화혈관내피세포삼여효천적염증궤제。
Objective To investigate possible role of 14 -3 -3 in the pathogenesis of asthma inflammation, the expression of 14 -3 -3 protein was observed in lung tissues of asthmatic rats.Methods Expressions of 14 -3 -3 protein was determined by immunohistochemisty method in lung tissues,and the relationship between 14 -3 -3 and asthma inflammation was analyzed.Results The location of positive expression of 14 -3 -3 protein was mainly at cytoplasm,while little at plasmalemma.The positive expression cell mostly was bronchial epithelial cell,others were lymphocytes,alveolar macrophages and vascular endothelial cells.On the other hand,the bronchial smooth muscle and vascular smooth muscle were negative expressed.Moreover,the expression level of 14 -3 -3 protein in asthma group [(0.353 ±0.023)absorbance]was significantly higher than that in the control group[(0.211 ±0.028 )absorbance] (t =10.969,P <0.01).Conclusion The results showed that the 14 -3 -3 protein was overexpressed in asthmatic lung tissue,it may play an important role in asthma inflammation through bronchial epithelial cells,lymphocytes, alveolar macrophages and vascular endothelial cell.