CAJ | 학술논문

目的：探讨甲钴胺对脑缺血再灌注大鼠脑组织细胞色素C表达的影响。方法选择Wistar大鼠132只，随机分为假手术组（24只）、模型组（27只）、尼莫地平组（27只）、甲钴胺低剂量组（27只）、甲钴胺高剂量组（27只）。采用改良线栓法栓塞大脑中动脉3 h制作大鼠脑缺血再灌注模型；分别于6、12、24 h用 TUNEL法检测大脑皮质细胞凋亡、Western blot检测大脑皮质细胞色素C蛋白的表达。结果尼莫地平组、甲钴胺低、高剂量组6、12、24 h阳性细胞数较模型组明显降低（ P＜0．01）；模型组6、12、24 h细胞色素C表达较假手术组明显升高（ P＜0．05，P＜0.01）；尼莫地平组、甲钴胺低、高剂量组6、12、24 h细胞色素C表达较模型组明显降低（ P＜0．05，P＜0．01）；甲钴胺高剂量组6、12 h细胞色素C表达较甲钴胺低剂量组明显降低（0．466±0．046 v s 0．617±0．033，P＜0．01；0．163±0.081 v s 0．552±0．132，P＜0．05）。结论甲钴胺对脑缺血再灌注损伤保护机制可能与抑制细胞色素C蛋白表达有关，以高剂量效果较好。
목적：탐토갑고알대뇌결혈재관주대서뇌조직세포색소C표체적영향。방법선택Wistar대서132지，수궤분위가수술조（24지）、모형조（27지）、니막지평조（27지）、갑고알저제량조（27지）、갑고알고제량조（27지）。채용개량선전법전새대뇌중동맥3 h제작대서뇌결혈재관주모형；분별우6、12、24 h용 TUNEL법검측대뇌피질세포조망、Western blot검측대뇌피질세포색소C단백적표체。결과니막지평조、갑고알저、고제량조6、12、24 h양성세포수교모형조명현강저（ P＜0．01）；모형조6、12、24 h세포색소C표체교가수술조명현승고（ P＜0．05，P＜0.01）；니막지평조、갑고알저、고제량조6、12、24 h세포색소C표체교모형조명현강저（ P＜0．05，P＜0．01）；갑고알고제량조6、12 h세포색소C표체교갑고알저제량조명현강저（0．466±0．046 v s 0．617±0．033，P＜0．01；0．163±0.081 v s 0．552±0．132，P＜0．05）。결론갑고알대뇌결혈재관주손상보호궤제가능여억제세포색소C단백표체유관，이고제량효과교호。
Objective To study the effect of methycobal on expression of cytochrome C in brain tis‐sue of rats following ischemia reperfusion (I/R) injury .Methods One hundred and thirty‐two Wistar rats were randomly divided into sham operation group (n=24) ,model group (n=27) ,ni‐modipine treatment group (n=27) ,low methycobal dose treatment group (n=27) ,high methyco‐bal dose treatment group (n=27) .A rat I/R injury model was established by Longa occlusion of the middle cerebral artery .Apoptosis and expression of cytochrome C in brain tissue of rats were detected by TUNEL and Western blot respectively at 6 ,12 ,24 h .Results The number of positive cells was significantly smaller in nimodipine treatment group ,low methycobal dose treatment group and high methycobal dose treatment group than in model group at 6 ,12 ,24 h (P<0 .01) . The expression level of cytochrome C was significantly higher in model group than in sham opera‐tion group ,significantly lower in nimodipine treatment group ,low methycobal dose treatment group and high methycobal dose treatment group than in model group at 6 ,12 ,24 h ( P<0 .05 , P<0 .01)and in high methycobal dose treatment group than in low methycobal dose treatment groupat6,12h(0.466±0.046 vs0.617±0.033,P<0.01 ;0.163±0.081 vs0.552±0.132,P<0 .05) .Conclusion Methycobal protects brain tissue of rats against I/R injury by downregulating the expression of cytochrome C .