CAJ | 학술논문

目的观察不同剂量的双醋瑞因对急性痛风性关节炎大鼠模型的防治作用并探讨其可能的机制.方法选取Wista大鼠30只,随机数字表法分为正常对照组、阴性(0.9％氯化钠注射液)对照组、阳性(秋水仙碱)对照组、双醋瑞因低剂量组、中剂量组和高剂量组.除正常对照组外,分别给予0.9％氯化钠注射液、秋水仙碱(0.3 mg·kg-1·d-1)、双醋瑞因(10、50及100 mg· kg-1·d-1灌胃8d,并于第6天制备急性痛风性关节炎模型.分别于造模后2、6、8、24及48 h观察各组大鼠关节肿胀情况及步态.48 h后处死大鼠,留取血清及关节组织浸出液,检测IL-1β和TNF-α水平.采用单因素方差分析、两两比较采用LSD-t检验和Kruskal-Wallis秩和检验进行数据分析.结果与正常对照组相比,阴性对照组大鼠造模后2h即出现明显关节肿胀,8h时达到高峰,之后逐渐下降,48 h后明显缓解.秋水仙碱组及双醋瑞因高剂量组关节肿胀情况[(0.106±0.081) cm和(0.112±0.030) cm]及踝关节炎症指数评分[(2.8±1.7)和(3.2±0.8)]在8h均较阴性对照组显著减轻[(0.208±0.078) cm,(4.7±1.6) cm;F=7.552,F=1.859,P均＜0.05].此外,与阴性对照组比较,双醋瑞因高剂量组大鼠步态明显好转(P＜0.05).秋水仙碱组和双醋瑞因高剂量组血清及关节浸出液中TNF-α及IL-1β水平均较照组明显降低.结论双醋瑞因各剂量组对痛风性关节炎大鼠都有一定的防治作用,且以高剂量组疗效为佳,其抗炎机制可能是通过下调前炎症介质IL-1β和TNF-α的表达来发挥作用的.
목적 관찰불동제량적쌍작서인대급성통풍성관절염대서모형적방치작용병탐토기가능적궤제.방법 선취Wista대서30지,수궤수자표법분위정상대조조、음성(0.9％록화납주사액)대조조、양성(추수선감)대조조、쌍작서인저제량조、중제량조화고제량조.제정상대조조외,분별급여0.9％록화납주사액、추수선감(0.3 mg·kg-1·d-1)、쌍작서인(10、50급100 mg· kg-1·d-1관위8d,병우제6천제비급성통풍성관절염모형.분별우조모후2、6、8、24급48 h관찰각조대서관절종창정황급보태.48 h후처사대서,류취혈청급관절조직침출액,검측IL-1β화TNF-α수평.채용단인소방차분석、량량비교채용LSD-t검험화Kruskal-Wallis질화검험진행수거분석.결과 여정상대조조상비,음성대조조대서조모후2h즉출현명현관절종창,8h시체도고봉,지후축점하강,48 h후명현완해.추수선감조급쌍작서인고제량조관절종창정황[(0.106±0.081) cm화(0.112±0.030) cm]급과관절염증지수평분[(2.8±1.7)화(3.2±0.8)]재8h균교음성대조조현저감경[(0.208±0.078) cm,(4.7±1.6) cm;F=7.552,F=1.859,P균＜0.05].차외,여음성대조조비교,쌍작서인고제량조대서보태명현호전(P＜0.05).추수선감조화쌍작서인고제량조혈청급관절침출액중TNF-α급IL-1β수평균교조조명현강저.결론 쌍작서인각제량조대통풍성관절염대서도유일정적방치작용,차이고제량조료효위가,기항염궤제가능시통과하조전염증개질IL-1β화TNF-α적표체래발휘작용적.
Objective To observe the preventive effect of different doses of diacerein on acute gout model rats and to explore its possible mechanism.Methods A total of 30 Wistar rats and were randomly assigned to the normal control group, the negative control (normal saline control group, and positive (colchicine) control group, diacerein low dose group, medium iddle dose group and high dose group.Except the normal control group that were given normal saline, other groups received colchicine (0.3 mg·kg-1 ·d-1), diacerein (10 mg·kg-1·d-1, 50 mg·kg-1·d-1 and 100 mg·kg-1·d-1) for 8 days respectively, and prepared acute gouty arthritis model on the 6th day, then observed rats joint swelling and gait at 2 h, 6 h, 8 h, 24 h and 48 h after the acute gouty arthritis model were established.After 48 h, the rats were killed, the serum and joint leachate of the rats were collected to test interleukin (IL)-1β and tumor necrosis factor (TNF)-α.One-way analysis of variance (ANOVA), LSD t test and Kruskal-Wallis rank sum test were used for statistical analysis.Results Compared with the normal group, joint swelling of the rats in the negative control group became significantly swollen at 2 h after the model was established,at and it reached the peak at 8 h reached the peak, then decreased gradually and remitted obviously at 48 h obviously remission.Joint swelling of the ccolchicine group and diacerein high dose group joint swelling [(0.106±0.081) cm, (0.112±0.030) cm] and ankle joint inflammation index score [(2.8±1.7), (3.16±0.75)] were significantly decreased compared with the negative control group [(0.208±0.078) cm, (4.7±1.6);P＜0.05].In addition, compared with the negative control group, the gait of diacerein high dose group was significantly improved (F=7.552, F=1.859, P＜0.05), colchieine group and diacerein high dose group, the serum and joint leaching liquid TNF-α and IL-1 levels also decreased significantly (P＜0.05).Conclusion Diacerein in any In each dosage group of has a certain preventive and therapeutic effect on rats gouty arthritis rats, especially in high dose group, the anti-inflammatory mechanism may be down-regulating the expression of proinflammatory mediators such as IL-1 beta and TNF-α.