中国癌症杂志
中國癌癥雜誌
중국암증잡지
China Oncology
2015年
10期
812-816
,共5页
骨桥蛋白%肺鳞状上皮细胞癌%淋巴结转移
骨橋蛋白%肺鱗狀上皮細胞癌%淋巴結轉移
골교단백%폐린상상피세포암%림파결전이
Osteopontin%Lung squamous cell carcinoma%Lymph node metastasis
背景与目的：骨桥蛋白(osteopontin，OPN)是一种分泌型磷酸化糖蛋白，在多种肿瘤组织中异常高表达，与肿瘤的发生、发展及预后有密切关系。当肿瘤细胞侵袭细胞外基质时，OPN可通过NF-κB依赖的信号转导通路促进肿瘤细胞高表达基质金属蛋白酶(matrix metal protease，MMPs)，引起细胞基底膜和细胞外基质降解，从而导致肿瘤发生浸润和转移。该研究通过检测肺鳞状上皮细胞癌(lung squamous cell carcinoma， SCC)患者肿瘤组织中OPN的蛋白水平，研究其与各临床指标的关系及其意义。方法：采用免疫组织化学染色法检测265例石蜡包埋的肺SCC组织和24例正常肺组织中OPN蛋白表达水平。结果：OPN在肺SCC组织表达阳性率为64.5%，显著高于正常肺组织(阳性率为29.2%，P<0.001)。OPN在肺SCC晚期(Ⅲ+Ⅳ期)表达阳性率为78.4%，显著高于早期(Ⅰ+Ⅱ期)组织(阳性率为54.5%，P<0.001)。在T3~4分期的肺SCC组织中OPN表达阳性率为76.9%，显著高于T1~2分期(59.4%，P=0.007)。OPN的表达与肺SCC的淋巴结转移情况显著相关，在有淋巴结转移的癌组织中表达阳性率为73.4%，高于无淋巴结转移的癌组织(阳性率为51.4%，P<0.001)。结论：OPN参与肺SCC的发生、发展和淋巴结转移，提示其在肺SCC的诊断、判断预后及治疗方面具有重要参考价值。
揹景與目的：骨橋蛋白(osteopontin，OPN)是一種分泌型燐痠化糖蛋白，在多種腫瘤組織中異常高錶達，與腫瘤的髮生、髮展及預後有密切關繫。噹腫瘤細胞侵襲細胞外基質時，OPN可通過NF-κB依賴的信號轉導通路促進腫瘤細胞高錶達基質金屬蛋白酶(matrix metal protease，MMPs)，引起細胞基底膜和細胞外基質降解，從而導緻腫瘤髮生浸潤和轉移。該研究通過檢測肺鱗狀上皮細胞癌(lung squamous cell carcinoma， SCC)患者腫瘤組織中OPN的蛋白水平，研究其與各臨床指標的關繫及其意義。方法：採用免疫組織化學染色法檢測265例石蠟包埋的肺SCC組織和24例正常肺組織中OPN蛋白錶達水平。結果：OPN在肺SCC組織錶達暘性率為64.5%，顯著高于正常肺組織(暘性率為29.2%，P<0.001)。OPN在肺SCC晚期(Ⅲ+Ⅳ期)錶達暘性率為78.4%，顯著高于早期(Ⅰ+Ⅱ期)組織(暘性率為54.5%，P<0.001)。在T3~4分期的肺SCC組織中OPN錶達暘性率為76.9%，顯著高于T1~2分期(59.4%，P=0.007)。OPN的錶達與肺SCC的淋巴結轉移情況顯著相關，在有淋巴結轉移的癌組織中錶達暘性率為73.4%，高于無淋巴結轉移的癌組織(暘性率為51.4%，P<0.001)。結論：OPN參與肺SCC的髮生、髮展和淋巴結轉移，提示其在肺SCC的診斷、判斷預後及治療方麵具有重要參攷價值。
배경여목적：골교단백(osteopontin，OPN)시일충분비형린산화당단백，재다충종류조직중이상고표체，여종류적발생、발전급예후유밀절관계。당종류세포침습세포외기질시，OPN가통과NF-κB의뢰적신호전도통로촉진종류세포고표체기질금속단백매(matrix metal protease，MMPs)，인기세포기저막화세포외기질강해，종이도치종류발생침윤화전이。해연구통과검측폐린상상피세포암(lung squamous cell carcinoma， SCC)환자종류조직중OPN적단백수평，연구기여각림상지표적관계급기의의。방법：채용면역조직화학염색법검측265례석사포매적폐SCC조직화24례정상폐조직중OPN단백표체수평。결과：OPN재폐SCC조직표체양성솔위64.5%，현저고우정상폐조직(양성솔위29.2%，P<0.001)。OPN재폐SCC만기(Ⅲ+Ⅳ기)표체양성솔위78.4%，현저고우조기(Ⅰ+Ⅱ기)조직(양성솔위54.5%，P<0.001)。재T3~4분기적폐SCC조직중OPN표체양성솔위76.9%，현저고우T1~2분기(59.4%，P=0.007)。OPN적표체여폐SCC적림파결전이정황현저상관，재유림파결전이적암조직중표체양성솔위73.4%，고우무림파결전이적암조직(양성솔위51.4%，P<0.001)。결론：OPN삼여폐SCC적발생、발전화림파결전이，제시기재폐SCC적진단、판단예후급치료방면구유중요삼고개치。
Background and purpose:Osteopontin (OPN) is a secreted glyco-phosphoprotein, which is overex-pressed by numerous human cancers, invovled in tumor occurrence, development and prognosis. OPN up-regulates matrix metal proteases (MMPs) expression in a NF-κB-dependent fashion during extracellular matrix (ECM) invasion causing degradation of cell basement membrane and ECM leading to tumor invasion and metastasis. The aim of this study was to examine the protein level of OPN in a large number of tissue samples from patients with lung squamous cell carcinoma (SCC), and evaluate its potential clinical value.Methods:The OPN protein levels in 265 tumor tissue samples and corresponding 24 normal lung tissue samples were examined by immunohistochemical (IHC) analysis.Results:IHC results showed that the positive rate of OPN was 64.5% in the SCC tissues, which was significantly higher than that in normal alveoli tissues (29.2%,P<0.001). The positive rate of OPN expression in late stage (Ⅲ+Ⅳ) tissues was 78.4%, significantly higher than that in early stage (Ⅰ+Ⅱ) tissue(positive rate 54.5%,P<0.001). The positive rate of OPN expression in T3-4 stage (Ⅲ+Ⅳ) tissue was 76.9%, significantly higher than that in T1-2 stage tissue (positive rate 59.4%,P=0.007). The expression of OPN was signifcantly correlated with the status of lymph node metastasis (LNM). The positive rate in the tumor tissue with LNM was 73.4%, significantly higher than that without (positive rate 51.4%,P<0.001).Conclusion:The level of OPN protein was overexpressed in lung cancer tissues, involved in SCC carcinogenesis and LNM. It is indicated that OPN has an impor-tant reference value in diagnosis, prognosis and therapy of SCC.
