CAJ | 학술논문

目的探讨高迁移率族蛋白1(HMGBl)、白细胞介素-6(IL-6)在癫(痫)患儿血清水平变化的意义.方法收集郑州大学第一附属医院6个月～12岁住院的癫(痫)患儿85例作为癫(痫)组,25例体检健康儿童作为健康对照组.其中癫(痫)组再分为癫(痫)频发组20例、癫(痫)持续状态组16例、非癫(痫)频发或持续状态组49例.采集癫(痫)发作后12 h内空腹外周静脉血,离心后备用,批量操作.采用双抗体夹心酶联免疫吸附法检测85例癫(痫)患儿及25例健康对照组儿童血清HMGB1及IL-6水平,采用SPSS 17.0软件对数据进行统计学分析.结果癫(痫)频发组、癫(痫)持续状态组、非癫(痫)频发或持续状态组及健康对照组血清中IL-6中位水平分别为14.559 ng/L、14.752 ng/L、11.981 ng/L、8.056 ng/L,HMGB1中位水平分别为8 621 ng/L、8 987 ng/L、7 870 ng/L、4221 ng/L.癫(痫)患儿血清中HMGB1及IL-6水平显著高于健康对照组,差异有统计学意义(P均＜0.05),癫(痫)频发组、癫(痫)持续状态组IL-6、HMGB1水平显著高于非癫(痫)频发或持续状态组,差异有统计学意义(P均＜0.05),癫(痫)频发组和癫(痫)持续状态组IL-6、HMGB1水平比较差异无统计学意义(P均＞0.05).IL-6水平与HMGB1呈正相关(r=0.760,P＜0.05).结论癫(痫)发作可以诱发炎性因子HMGB1及IL-6高表达,给予HMGB1及IL-6抑制剂可能有利于癫(痫)的控制.
목적 탐토고천이솔족단백1(HMGBl)、백세포개소-6(IL-6)재전(간)환인혈청수평변화적의의.방법 수집정주대학제일부속의원6개월～12세주원적전(간)환인85례작위전(간)조,25례체검건강인동작위건강대조조.기중전(간)조재분위전(간)빈발조20례、전(간)지속상태조16례、비전(간)빈발혹지속상태조49례.채집전(간)발작후12 h내공복외주정맥혈,리심후비용,비량조작.채용쌍항체협심매련면역흡부법검측85례전(간)환인급25례건강대조조인동혈청HMGB1급IL-6수평,채용SPSS 17.0연건대수거진행통계학분석.결과 전(간)빈발조、전(간)지속상태조、비전(간)빈발혹지속상태조급건강대조조혈청중IL-6중위수평분별위14.559 ng/L、14.752 ng/L、11.981 ng/L、8.056 ng/L,HMGB1중위수평분별위8 621 ng/L、8 987 ng/L、7 870 ng/L、4221 ng/L.전(간)환인혈청중HMGB1급IL-6수평현저고우건강대조조,차이유통계학의의(P균＜0.05),전(간)빈발조、전(간)지속상태조IL-6、HMGB1수평현저고우비전(간)빈발혹지속상태조,차이유통계학의의(P균＜0.05),전(간)빈발조화전(간)지속상태조IL-6、HMGB1수평비교차이무통계학의의(P균＞0.05).IL-6수평여HMGB1정정상관(r=0.760,P＜0.05).결론 전(간)발작가이유발염성인자HMGB1급IL-6고표체,급여HMGB1급IL-6억제제가능유리우전(간)적공제.
Objective To explore the changes of high mobility protein group box 1 (HMGB1) and interleukin-6 (IL-6) in epileptic patients and discuss the significance.Methods Eighty-five children aged 6 months to 12 years old with idiopathic generalized or partial epilepsy diagnosed in the First Affiliated Hospital of Zhengzhou University as the epileptic group,and it consisted of 20 cases of frequent epilepsy subgroup, 16 cases of status epilepticus subgroup, and 49 cases of non frequent or status epilepticus subgroup.Twenty-five healthy children in outpatient department were selected as the healthy controls.Fasting veinal blood was obtained after seizure within 12 hours.The serum was collected and kept in the-80 ℃ refrigerator.Enzyme linked immunosorbent assay was adopted to investigate the levels of HMGB1 and IL-6 in the serum.The data were analyzed by using SPSS 17.0 software.Results The levels of IL-6 in the serum of frequent epilepsy subgroup, status epilepticus subgroup, non frequent or status epilepticus subgroup and the healthy controls were 14.559 ng/L, 14.752 ng/L, 11.981 ng/L, 8.056 ng/L, respectively, and the levels of HMGB1 were 8 621 ng/L,8 987 ng/L,7 870 ng/L,4 221 ng/L,respectively.The levels of IL-6,HMGB1 of epileptic groups were higher than the healthy controls, especial the levels of IL-6, HMGB1 of frequent epilepsy subgroup and the status epilepticus subgroup were higher than other in non frequent or status epilepticus subgroup,but the levels of IL-6, HMGB1 between epilepsy subgroup and status epilepticus subgroup had no obvious difference(P ＞0.05).The level of IL-6 had a positive correlation with level of HMGB1 (r =0.760, P ＜ 0.05).Conclusions It suggests that HMGB1 and the cytokine network may contribute to the generation of epilepsy in children.The inhibitor of HMGB1 and IL-6 play a potential role in treatment of epilepsy.