CAJ | 학술논문

针对现有凝胶图像间蛋白点匹配方法在选择特征时，没有直观和统一标准的问题，本文提出了基于相似度图的蛋白点特征分析方法。首先给出相似度图的定义和生成方法；其次利用相似度图法分析坐标相似度、形状上下文相似度和形态参数相似度等5种常用特征的特点及优劣；最后根据特征分析结果提出一种多特征综合利用的乘积法，相比均值法具有更好的匹配效果。为验证相似度图和多特征综合乘积法的有效性，开展了多种图源的凝胶图像蛋白点匹配实验，结果表明，相似度图能够直观、有效地反映蛋白点特征的匹配性能，对多特征的选择和综合利用具有很好的指导意义。
침대현유응효도상간단백점필배방법재선택특정시，몰유직관화통일표준적문제，본문제출료기우상사도도적단백점특정분석방법。수선급출상사도도적정의화생성방법；기차이용상사도도법분석좌표상사도、형상상하문상사도화형태삼수상사도등5충상용특정적특점급우렬；최후근거특정분석결과제출일충다특정종합이용적승적법，상비균치법구유경호적필배효과。위험증상사도도화다특정종합승적법적유효성，개전료다충도원적응효도상단백점필배실험，결과표명，상사도도능구직관、유효지반영단백점특정적필배성능，대다특정적선택화종합이용구유흔호적지도의의。
There are no intuitive and unified standards for selecting features in existing protein gel image point matching method, for which an analysis method based on similarity map is proposed. Firstly, the definition and generation methods of similarity map were presented. Secondly, trait and merits of features such as coordinate similarity, shape-context similarity and morphology similarity were analyzed using similarity map method. Finally, comprehensive utilization of multi-features named product-method which has a better effect than mean-method was proposed based on the results. Many experiments using different 2-DE gel images were carried out to prove the validity of similarity map and product-method. The results showed that similarity map could be used for intuitional, effective analysis of matching performance and to guide the selection and comprehensive utilization of multi-features.