CAJ | 학술논문

对非洲凤仙(Impatiens walleriana Hook.f.)进行染色体研究,旨在为多倍体育种提供细胞学资料.以非洲凤仙根尖为材料,采用常规压片法制片,光学显微镜观察染色体. 结果表明,根尖8:00~9:00取材、在4℃下0.002 mol/L的8-羟基喹啉预处理2 h的处理组合效果最佳. 间期核由一些大大小小的染色较深的异染色质颗粒构成,间期核属于前染色体型. 前期染色体由染色较深的异固缩节段和染色较浅的常染色质节段相间排列而成,较多地分布于细胞中央,前期染色体属于渐变型. 中期染色体数目2n=16,为二倍体.核型公式为:2n=2x=16=14m+2sm,其中第一对为近中部着丝点染色体,其余均为中部着丝点染色体,未观察到随体. 最长染色体与最短染色体的比值为1.56,臂比大于2的染色体占12.5%,核型属2A型,核型不对称系数为54.8%,为对称的核型. 该种的核型为首次报道.
대비주봉선(Impatiens walleriana Hook.f.)진행염색체연구,지재위다배체육충제공세포학자료.이비주봉선근첨위재료,채용상규압편법제편,광학현미경관찰염색체. 결과표명,근첨8:00~9:00취재、재4℃하0.002 mol/L적8-간기규람예처리2 h적처리조합효과최가. 간기핵유일사대대소소적염색교심적이염색질과립구성,간기핵속우전염색체형. 전기염색체유염색교심적이고축절단화염색교천적상염색질절단상간배렬이성,교다지분포우세포중앙,전기염색체속우점변형. 중기염색체수목2n=16,위이배체.핵형공식위:2n=2x=16=14m+2sm,기중제일대위근중부착사점염색체,기여균위중부착사점염색체,미관찰도수체. 최장염색체여최단염색체적비치위1.56,비비대우2적염색체점12.5%,핵형속2A형,핵형불대칭계수위54.8%,위대칭적핵형. 해충적핵형위수차보도.
In order to provide necessary cytology information on polyploid breeding,the cytology of Impatiens walleriana Hook.f. had been studied by the conventional squashing method. The results showed that the optional root sampling time was 8:00~9:00, 0.002 mol/L 8-Hydroxyquinoline for 2 h at 4℃ had better effect; the interphase nuclei and prophase chromosomes were found to be of the prochromosome type and the interstitial type, respectively. The number of the somatic chromosome was 16, and it was diploid. The karyotype formulae was 2n=2x=16=14m+2sm. The first pair of chromosomes were submetacentric, and oth-ers were metacentric, there was no observed satellite. The ratio of chromosome length, i.e., ratio of the length of the longest chromosome to that of the shortest one was 1.56. The percentage of chromosome with arm ratio beyond 2 was 12.5%. The karyotype was 2A and the AsK was 54.8%. The karyotype was reported for the first time.