CAJ | 학술논문

目的：研究吡格列酮(Pioglitazone，Pio)对Aβ25~35引起的大鼠学习记忆能力和海马内氧化应激反应的影响。方法将40只大鼠随机分为空白对照组、模型组、Pio低剂量组和Pio高剂量组，每组10只。Pio低剂量组和高剂量组大鼠分别给予吡格列酮40 mg/kg和80 mg/kg灌胃21 d，空白对照组和模型组给予等量生理盐水灌胃。治疗结束次日进行Morris水迷宫实验，前5 d定位航行训练，第6天进行空间探索实验。Morris水迷宫实验结束后次日，麻醉、断头取脑，提取脑组织匀浆，以备ELISA实验使用。ELISA法检测大鼠海马内谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量。结果 Morris水迷宫结果显示，模型组大鼠潜伏期比空白对照组明显延长，而穿过平台次数和平台滞留时间缩短，差异均具有统计学意义(P<0.05）；与模型组相比，Pio低剂量和高剂量组大鼠潜伏期显著降低，穿过平台次数和滞留时间也相应增加，差异均具有统计学意义(P<0.05)，其中Pio低剂量组增加显著；ELISA法检测结果显示，模型组大鼠海马内GSH-Px和SOD含量比空白对照组明显降低，而脂质过氧化产物MDA明显增加，差异均具有统计学意义(P<0.05)；与模型组相比，Pio低剂量和高剂量组大鼠海马内GSH-Px和SOD的活性明显升高，MDA明显减少，差异均具有统计学意义(P<0.05)，其中Pio低剂量组增加显著。结论吡格列酮能够改善痴呆大鼠的学习记忆能力，其机制可能与对抗海马内的氧化应激反应有关。
목적：연구필격렬동(Pioglitazone，Pio)대Aβ25~35인기적대서학습기억능력화해마내양화응격반응적영향。방법장40지대서수궤분위공백대조조、모형조、Pio저제량조화Pio고제량조，매조10지。Pio저제량조화고제량조대서분별급여필격렬동40 mg/kg화80 mg/kg관위21 d，공백대조조화모형조급여등량생리염수관위。치료결속차일진행Morris수미궁실험，전5 d정위항행훈련，제6천진행공간탐색실험。Morris수미궁실험결속후차일，마취、단두취뇌，제취뇌조직균장，이비ELISA실험사용。ELISA법검측대서해마내곡광감태과양화물매(GSH-Px)、초양화물기화매(SOD)적활성급병이철(MDA)적함량。결과 Morris수미궁결과현시，모형조대서잠복기비공백대조조명현연장，이천과평태차수화평태체류시간축단，차이균구유통계학의의(P<0.05）；여모형조상비，Pio저제량화고제량조대서잠복기현저강저，천과평태차수화체류시간야상응증가，차이균구유통계학의의(P<0.05)，기중Pio저제량조증가현저；ELISA법검측결과현시，모형조대서해마내GSH-Px화SOD함량비공백대조조명현강저，이지질과양화산물MDA명현증가，차이균구유통계학의의(P<0.05)；여모형조상비，Pio저제량화고제량조대서해마내GSH-Px화SOD적활성명현승고，MDA명현감소，차이균구유통계학의의(P<0.05)，기중Pio저제량조증가현저。결론필격렬동능구개선치태대서적학습기억능력，기궤제가능여대항해마내적양화응격반응유관。
Objective To study the effects of pioglitazone (Pio) on learning and memory abilities and oxida-tive stress in the hippocampus of rats with Aβ25~35-induced Alzheimer's disease. Methods Forty rats were randomly divided into four groups: control group, model group, low-dose Pio group and high-dose Pio group, with 10 rats in each group. And the rats in low-dose Pio group and high-dose Pio group were administrated with Pio of 40 mg/kg and 80 mg/kg, respectively, for 21 days. The control group and model group were administrated with the same volume of normal saline. Morris water maze test was used to test the learning and memory abilities of the rats. During the first 5 days, orientation navigation test was carried out, followed by the spatial probe test on the sixth day. After the behavior test, the rats were sacrificed for preparing tissue homogenate. And ELISA was used to measure glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, as well as the level of maleic dialdehyde (MDA). Results Mor-ris water maze test showed that the escape latency was significantly prolonged in rats of model group compared with control group, while the number of crossing the platform and time spent on the platform were reduced (P<0.05). After administration of Pio, the escape latency was significantly decreased, while the number of crossing the platform and time spent on the platform were increased significantly, particularly in low-dose Pio group, as compared with model group (P<0.05). ELISA showed that compared with the control group, the activities of GSH-Px and SOD were de-creased but the level of MDA was increased in the AD rats of model group (P<0.05). Pio significantly increased the ac-tivities of GSH-Px and SOD but decreased the level of MDA in the AD rats, especially in low-dose Pio group (P<0.05). Conclusion Pio can improve the learning and memory abilities of AD rats, the mechanism of which may be related to attenuating oxidative stress in the hippocampus.