湖北农业科学
湖北農業科學
호북농업과학
Hubei Agricultural Sciences
2015年
21期
5364-5367
,共4页
陈皮%橘皮素%川陈皮素%正交试验%工艺
陳皮%橘皮素%川陳皮素%正交試驗%工藝
진피%귤피소%천진피소%정교시험%공예
tangerine peel%tangeretin%nobiletin%orthogonal design%extraction technology
橘皮素和川陈皮素是陈皮多甲氧基黄酮的重要活性成分,以HPLC法测定陈皮提取物中橘皮素和川陈皮素的含量,优选了提取溶剂,设计正交试验考察了提取温度、提取时间、溶剂添加量和提取次数对提取工艺的影响,确定了最佳提取工艺. 结果表明,石油醚是同时提取橘皮素和川陈皮素的适宜溶剂,正交试验所得的最佳提取工艺为加入6倍量石油醚,80℃提取4次,每次3 h,所获得的提取工艺合理、稳定、可靠.
橘皮素和川陳皮素是陳皮多甲氧基黃酮的重要活性成分,以HPLC法測定陳皮提取物中橘皮素和川陳皮素的含量,優選瞭提取溶劑,設計正交試驗攷察瞭提取溫度、提取時間、溶劑添加量和提取次數對提取工藝的影響,確定瞭最佳提取工藝. 結果錶明,石油醚是同時提取橘皮素和川陳皮素的適宜溶劑,正交試驗所得的最佳提取工藝為加入6倍量石油醚,80℃提取4次,每次3 h,所穫得的提取工藝閤理、穩定、可靠.
귤피소화천진피소시진피다갑양기황동적중요활성성분,이HPLC법측정진피제취물중귤피소화천진피소적함량,우선료제취용제,설계정교시험고찰료제취온도、제취시간、용제첨가량화제취차수대제취공예적영향,학정료최가제취공예. 결과표명,석유미시동시제취귤피소화천진피소적괄의용제,정교시험소득적최가제취공예위가입6배량석유미,80℃제취4차,매차3 h,소획득적제취공예합리、은정、가고.
Tangeretin and nobiletin were important polymethoxyflavones in tangerine peel. The content of tangeretin and no-biletin were measured by HPLC methods. The extracting solvents were selected. The influence of extracting temperature, ex-tracting time, the menstruum dosage, extracting times were investigated using orthogonal design method. The results showed that petroleum ether was the best extracting solvent. The optimal conditions for the extraction technology were as follows: ex-tracting for four times, each time for 3 h at 80℃, with 6 times the amount of petroleum ether. This extraction technology is reasonable, stable and feasible.
