CAJ | 학술논문

采用不连续垂直板聚丙烯酰胺凝胶电泳法,对连续自交四代和开放授粉的4个甘蓝型油菜品系叶片过氧化物酶(POD)和酯酶(EST)同工酶进行酶谱分析.结果表明,4个品系开放授粉POD同工酶活性强于各自交世代;EST同工酶酶带除品系03武240-1开放授粉的酶活性较其自交世代弱外,其余3个均强于自交世代,说明连续自交会使甘蓝型油菜后代的生活力减弱,但品系间有差异.
채용불련속수직판취병희선알응효전영법,대련속자교사대화개방수분적4개감람형유채품계협편과양화물매(POD)화지매(EST)동공매진행매보분석.결과표명,4개품계개방수분POD동공매활성강우각자교세대;EST동공매매대제품계03무240-1개방수분적매활성교기자교세대약외,기여3개균강우자교세대,설명련속자교회사감람형유채후대적생활력감약,단품계간유차이.
Using vertical plate discontinuous polyacrylamide gel electrophoresis,analysis the leaf peroxidase (POD) and esterase (EST)isozyme for four successive selfing generations and open pollination four Brassica napus L. . The result shows that the POD isozyme of the open pollinated are stronger than self-generations;the EST isozyme of the open pollinated are stronger than self-generations in addition to 03 Wu 240-1,Successive selfing led to reduction of viability of Brassica napus L.,but there are differences between strains.