CAJ | 학술논문

目的：观察研究人参皂苷Rh2对糖尿病大鼠心肌损伤的改善作用,并初步探讨其对糖尿病大鼠心肌氧化应激的影响,为DCM的临床防治提供新的手段.方法通过形态观察、心功能测定以及应用生物试剂盒和ELISA方法,检测观察STZ诱发的DM大鼠血清和心肌组织,主要研究下列问题：1)人参皂苷Rh2对DM大鼠心肌的保护作用；2)人参皂苷Rh2对DM大鼠心肌损伤过程中氧化应激作用的影响.结果人参皂苷Rh2可降低血清及心肌组织MDA含量,降低心肌组织8-OHdG水平,提高血清和心肌组织抗氧化酶SOD,CAT和GSH-Px活性(P<0.05).结论人参皂苷Rh2对DM大鼠心肌具有保护作用,其改善机制部分是通过清除大鼠心肌组织内ROS、提高抗氧化酶活性、降低大鼠体内氧化应激水平而实现的.
목적：관찰연구인삼조감Rh2대당뇨병대서심기손상적개선작용,병초보탐토기대당뇨병대서심기양화응격적영향,위DCM적림상방치제공신적수단.방법통과형태관찰、심공능측정이급응용생물시제합화ELISA방법,검측관찰STZ유발적DM대서혈청화심기조직,주요연구하렬문제：1)인삼조감Rh2대DM대서심기적보호작용；2)인삼조감Rh2대DM대서심기손상과정중양화응격작용적영향.결과인삼조감Rh2가강저혈청급심기조직MDA함량,강저심기조직8-OHdG수평,제고혈청화심기조직항양화매SOD,CAT화GSH-Px활성(P<0.05).결론인삼조감Rh2대DM대서심기구유보호작용,기개선궤제부분시통과청제대서심기조직내ROS、제고항양화매활성、강저대서체내양화응격수평이실현적.
Objective To study the effect of ginsenoside Rh2 on myocardial injury in diabetic rats,to explore its influence on oxidative stress in diabetic rats,and to provide a new method for clinical prevention and treatment of DCM. Method The serum indexes and myocardial tissues of the diabetic rats induced by STZ were detected and observed through morphological observation, cardiac function measurement, application of biological reagent kit and ELISA detection method. The following questions were investigated:1 ) the protective effects of ginsenoside Rh2 on myocardium in diabetic rats;2 ) the influences of ginsenoside Rh2 on oxidative stress in the process of myocardial injury in diabetic rats. Results Ginsenoside Rh2 could decrease the content of MDA in serum and myocardium,reduce the level of 8-OHdG in myocardial tissue,improve the activities of antioxidant enzymes SOD, CAT and GSH-Px in serum and myocardium(P<0. 05). Conclusion Ginsenoside Rh2 has protective effect on myocardium in diabetic rats. Its improvement mechanism may enhance the activity of antioxidant enzyme, and reduce the level of oxidative stress in rats through the clearance of ROS in myocardium.