中南大学学报(英文版)
中南大學學報(英文版)
중남대학학보(영문판)
Journal of Central South University
2015年
12期
4528-4535
,共8页
陈勃伟%武彪%刘兴宇%温建康
陳勃偉%武彪%劉興宇%溫建康
진발위%무표%류흥우%온건강
bioleaching%CO2%N2%chalcocite%pyrite%microbial community
Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40 °C with aeration of CO2 and N2. Ore samples were collected at day 43, 64, 85, 106 and subjected to microbial community analysis by 16S rRNA gene clone library. Phylogenetic analyses of 16S rDNA fragments revealed that the retrieved sequences are mainly related to genus Acidithiobacillus,Leptospirillum andSulfobacillus. Aeration of CO2 and N2 significantly impacted the microbial community composition. When CO2 was aerated, the proportion of genusAcidithiobacillus considerably increased, whereas the proportion of genusLeptospirillum and genusSulfobacillus declined. However, with the aeration of N2, the proportion of genusAcidithiobacillus andLeptospirillum increased, but genusSulfobacillus decreased. When there was no aeration, the microbial community was similar to the inocula with the proportion of genusLeptospirillum mounted. These results indicated that the limitation of oxygen could change the bioleaching microbial community and the aeration of CO2 and N2 was favourable for the growth of sulfur-oxidizer (At. caldus) and iron-oxidizer (L. ferriphilum) respectively, which could be used for the regulation of microorganisms’ role in mineral bioleaching.