CAJ | 학술논문

目的：本项目旨在探讨在体外实验中洛伐他汀（Lovastatin，LV）是否能够通过增加对化疗药物的敏感性而提高对乳腺癌细胞的杀伤效果。方法：采用超低粘附悬浮培养法从MDA-MB-231细胞获得乳腺癌干细胞样细胞。用LV加或不加化疗药物在缺氧条件下对细胞进行处理。用流式细胞仪检测细胞凋亡，用化学发光法检测细胞Caspase 3/7活性，用MTT法检测细胞增殖活性。结果：单独使用时，LV能够显著促进MDA-MB-231细胞的凋亡并增加Caspase 3/7活性，而对ER阳性的MCF-7作用不明显。与化疗药物多柔比星联合使用时，LV显著增加乳腺癌干细胞对多柔比星的敏感性。结论：天然化合物LV在体外能够促进乳腺癌细胞凋亡并增加乳腺癌干细胞对化疗药物多柔比星的敏感性。上述研究结果为设计针对乳腺癌的治疗方案提供了一种可能的选择，其体内作用仍需进一步研究证实。
목적：본항목지재탐토재체외실험중락벌타정（Lovastatin，LV）시부능구통과증가대화료약물적민감성이제고대유선암세포적살상효과。방법：채용초저점부현부배양법종MDA-MB-231세포획득유선암간세포양세포。용LV가혹불가화료약물재결양조건하대세포진행처리。용류식세포의검측세포조망，용화학발광법검측세포Caspase 3/7활성，용MTT법검측세포증식활성。결과：단독사용시，LV능구현저촉진MDA-MB-231세포적조망병증가Caspase 3/7활성，이대ER양성적MCF-7작용불명현。여화료약물다유비성연합사용시，LV현저증가유선암간세포대다유비성적민감성。결론：천연화합물LV재체외능구촉진유선암세포조망병증가유선암간세포대화료약물다유비성적민감성。상술연구결과위설계침대유선암적치료방안제공료일충가능적선택，기체내작용잉수진일보연구증실。
ObjectiveTo explore whether lovastatin (LV) can improve the cytotoxic effect of the chemotherapeutic drug Doxorubicin on breast cancer cells in vitro.Methods Breast cancer stem cells were obtained by ultra-low adherence suspen-sion culture from MDA-MB-231 cells. The cells were treated with LV in combination with or without Doxorubicin under hy-poxia. Cell apoptosis, Caspase 3/7 activity and cell proliferation were detected by flow cytometry, bioluminescent ELISA assay and MTT assay, respectively.Results When used alone, LV could significantly promote apoptosis and increase the Caspase 3/7 activity of MDA-MB-231 parental cells while having little effect on ER-positive MCF-7 cells. When used in combination with Doxorubicin, LV significantly enhanced the sensitivity of MDA-MB-231 stem cells to Doxorubicin.Conclusion The natural compound LV promotes apoptosis of highly malignant MDA-MB-231 breast cancer cels and increases the sensitivity of MDA-MB-231 stem cels to the chemotherapeutic drug Doxorubicin. Although further studies are needed to confirm the in vivo effects of LV, these preliminary results provide a rationale for designing better treatment modality for breast cancer cells.