CAJ | 학술논문

为明确罗汉果新病害斑枯病的病原菌种类并筛选出有效的防治药剂,采用组织分离法分离病原菌并通过柯赫氏法则验证其致病性,根据菌落特征、菌株形态及 ITS 序列分析进行病原菌的鉴定；采用生长速率法测定了12种杀菌剂对病原菌的室内抑制活性。结果表明,罗汉果新病害的病原菌为 Stagonosporopsis cucurbitacearum ；供试的药剂中,60%咪鲜胺锰盐 WP(0.5 g/L)、80%戊唑醇 WP(0.25 g/L)、45%甲霜·恶霉灵 WP(1 g/L)、60%唑醚·代森联 WP (0.5 g/L)室内对该菌菌丝的抑制率均达100%,可进一步进行大田药效试验,将药效好的药剂推荐田间使用。
위명학라한과신병해반고병적병원균충류병사선출유효적방치약제,채용조직분리법분리병원균병통과가혁씨법칙험증기치병성,근거균락특정、균주형태급 ITS 서렬분석진행병원균적감정；채용생장속솔법측정료12충살균제대병원균적실내억제활성。결과표명,라한과신병해적병원균위 Stagonosporopsis cucurbitacearum ；공시적약제중,60%미선알맹염 WP(0.5 g/L)、80%무서순 WP(0.25 g/L)、45%갑상·악매령 WP(1 g/L)、60%서미·대삼련 WP (0.5 g/L)실내대해균균사적억제솔균체100%,가진일보진행대전약효시험,장약효호적약제추천전간사용。
A new disease in Siraitia grosvenorii occurred seriously in producing area of Guangxi.The fungus was isolated from infected plants according to Koch’s Rule.Based on morphological characters and molecular analysis of internal transcribed spacer (ITS)sequences,the pathogen was identified to be Stagonosporopsis cucurbita-cearum .Antimicrobial activity of 12 fungicides by mycelial growth rate method indicated that 60% prochloraz-manganese chloride complex WP(0.5 g/L),80% tebuconazole WP(0.25 g/L),45% metalaxyl·hymexazol WP (1 g/L)and the 60% pyraclostrobin·metiram WP (0.5 g/L)had strong antimicrobial effects,with the inhibito-ry ratio of 100%.This is the first report of S .cucurbitacearum infecting S .grosvenorii worldwide.The fungicides should be further tested in the field to control the new disease.