CAJ | 학술논문

通过考察短梗霉素 A(AbA)对灰葡萄孢菌野生株 BcAUR1及其 AUR1基因内含子缺失突变株 BcAUR1a 生长的影响,明确 AbA 抑制真菌生长的机理。AbA 敏感性试验表明,低浓度 AbA (8μg/mL)显著抑制野生株BcAUR1菌体的生长,高浓度 AbA(50μg/mL)存在下观察不到 BcAUR1的生长迹象。突变株 BcAUR1a 则不受AbA 的影响,在低浓度和高浓度 AbA 存在下均能正常生长。AbA 抑制 BcAUR1侵染柑橘果实,但 BcAUR1a 在高浓度 AbA 存在下也能够有效感染柑橘果实。这两个试验均证实了突变株 BcAUR1a 具有 AbA 抗性。电镜观察表明,AbA 引起 BcAUR1细胞质膜和内膜系统形态异常,质膜和液泡膜断裂,细胞内物质泄露。AbA 抑制灰葡萄孢菌生长的机制是由于 IPC 合成酶受到抑制,导致鞘磷脂类物质合成不足,细胞膜结构破坏,胞内物质外漏。
통과고찰단경매소 A(AbA)대회포도포균야생주 BcAUR1급기 AUR1기인내함자결실돌변주 BcAUR1a 생장적영향,명학 AbA 억제진균생장적궤리。AbA 민감성시험표명,저농도 AbA (8μg/mL)현저억제야생주BcAUR1균체적생장,고농도 AbA(50μg/mL)존재하관찰불도 BcAUR1적생장적상。돌변주 BcAUR1a 칙불수AbA 적영향,재저농도화고농도 AbA 존재하균능정상생장。AbA 억제 BcAUR1침염감귤과실,단 BcAUR1a 재고농도 AbA 존재하야능구유효감염감귤과실。저량개시험균증실료돌변주 BcAUR1a 구유 AbA 항성。전경관찰표명,AbA 인기 BcAUR1세포질막화내막계통형태이상,질막화액포막단렬,세포내물질설로。AbA 억제회포도포균생장적궤제시유우 IPC 합성매수도억제,도치초린지류물질합성불족,세포막결구파배,포내물질외루。
In order to clarify the mechanism of aureobasidin A(AbA)inhibiting the growth of fungi,the effects of AbA on the growth of B .cinerea wild strain BcAUR1 and strain BcAUR1a with deletion of the intron of AUR 1 gene were investigated.The experiments of AbA sensitivity showed that low concentration of AbA (8 μg/mL)sig-nificantly inhibited the growth of BcAUR1,and there was no sign of BcAUR1 growth under high concentrations of AbA (50 μg/mL).However,AbA had no impacts on BcAUR1a,which could grow normally at low or high concentrations of AbA.Additionally,AbA could inhibit infection of BcAUR1 to citrus fruits,but BcAUR1a could infect citrus fruits effectively even at the presence of high concentration of AbA.Both results confirmed that BcAUR1a had a resistance to AbA.The observation by electron microscopy showed that AbA caused morphologi-cal abnormality of BcAUR1 cell membrane and inner membrane system,the fracture of plasmalemma and tono-plast,and the leakage of intracellular substances.The mechanisms that AbA inhibited the growth of B .cinerea was that IPC synthetase was inhibited,which caused insufficient synthesis of sphingomyelin substances,damage of cell membrane structure and leakage of intracellular substances.