CAJ | 학술논문

本研究采用两种人工接种方法,以玉米成株期病情指数为指标,鉴定了63个转基因玉米株系的田间抗病性。结果表明,T4代转化株系‘47’和‘13h2-3’的抗病性达 R 抗级,超过抗病对照‘H9-21’。有82.5%(52/63)的转基因株系抗病性比未转化对照显著提高,表明用反向重复的 RNA 干涉表达载体转化玉米,可获得转基因抗病毒材料,且干涉片段适度延长可增加获得抗病材料的几率。另外,本文通过两种病毒接种方法的比较,发现采用玻璃纤维刷苗床穿刺接种比常规石英砂摩擦接种具有更小的误差。
본연구채용량충인공접충방법,이옥미성주기병정지수위지표,감정료63개전기인옥미주계적전간항병성。결과표명,T4대전화주계‘47’화‘13h2-3’적항병성체 R 항급,초과항병대조‘H9-21’。유82.5%(52/63)적전기인주계항병성비미전화대조현저제고,표명용반향중복적 RNA 간섭표체재체전화옥미,가획득전기인항병독재료,차간섭편단괄도연장가증가획득항병재료적궤솔。령외,본문통과량충병독접충방법적비교,발현채용파리섬유쇄묘상천자접충비상규석영사마찰접충구유경소적오차。
Resistance of the 63 transformed lines was evaluated by disease index of maize at adult stage in field ex-periment by two artificial inoculation methods.The results showed that the resistant grade of the T4 transformed lines ‘47’and ‘13h2-3’was R grade,higher than that of the resistant control ‘H9-21’,and 82.5%(52/63)of the transformed lines had significantly higher resistance than non-transformed control lines.It is concluded that the transformation by reverse repeated RNA-interference expression vector is an effective method to obtain trans-formed materials for virus resistance.The resistance will be improved if the interference fragment has an appro-priate length.In addition,it was found that puncturing inoculation by glass fiber brush in seedbed was of less devi-ation than conventional inoculation by quartz sand friction.