CAJ | 학술논문

建立测定一次性纸杯和餐盒中荧光增白剂含量的高效液相色谱-荧光方法。采用Inertsil ODS-3(250 mm ×4.6 mm,5μm)为分析柱,以5 mmol/L乙酸铵溶液和乙腈为流动相,梯度洗脱,流速为0.5 mL/min,荧光激发波长为350 nm,发射波长为430 nm。结果表明,所选用的3种荧光增白剂(B2729、220、B2730)可以较好地被分离,回收率为77.4%~94.6%,相对标准偏差<10%且线性关系良好。该法简便快捷、结果准确、灵敏度高,能够满足对一次性纸杯和餐盒中荧光增白剂含量的检测。
건립측정일차성지배화찬합중형광증백제함량적고효액상색보-형광방법。채용Inertsil ODS-3(250 mm ×4.6 mm,5μm)위분석주,이5 mmol/L을산안용액화을정위류동상,제도세탈,류속위0.5 mL/min,형광격발파장위350 nm,발사파장위430 nm。결과표명,소선용적3충형광증백제(B2729、220、B2730)가이교호지피분리,회수솔위77.4%~94.6%,상대표준편차<10%차선성관계량호。해법간편쾌첩、결과준학、령민도고,능구만족대일차성지배화찬합중형광증백제함량적검측。
A method for the determination of fluorescent whitening agents in disposable cups and contain-ers by high performance liquid chromatography ( HPLC) with fluorescence detector was developed. The HPLC method was performed on a column of Inertsil ODS-3(250 mm × 4. 6 mm,5 μm) by gradient elu-tion using 5 mmol/L ammonium acetate and acetonitrile as the mobile phases with a flow rate of 0. 5 mL/min,and detected by the fluorescence detector at an excitation wavelength of 350 nm and an emission wavelength of 430 nm. Results showed that the three fluorescent whitening agents( B2729,220, B2730) were separated well. The recovery were 77. 4% ~94. 6%,good linearities with RSD were not more than 10%. The method is simple,accurate,sensitive and can meet the requirements of the determi-nation of fluorescent whitening agents in disposable cups and containers.