植物保护
植物保護
식물보호
Plant Protection
2015年
6期
1-8
,共8页
植物线虫%分子鉴定%DNA%PCR
植物線蟲%分子鑒定%DNA%PCR
식물선충%분자감정%DNA%PCR
plant nematode%molecular identification%DNA%PCR
植物线虫可危害农作物和林木，对它们的准确鉴定是防治植物线虫病害的基础。由于植物线虫很小，而且在形态上种间常有覆盖，而种内有较大的变异，仅依据形态特征很难鉴定。分子鉴定技术给植物线虫的检测和鉴定提供了快速、精确、可靠的方法。文章综述了植物线虫分子的 DNA 提取、分子鉴定靶标序列的选择及分子鉴定方法等方面的研究进展及现状，以促进对植物线虫分子鉴定更深入的研究。
植物線蟲可危害農作物和林木，對它們的準確鑒定是防治植物線蟲病害的基礎。由于植物線蟲很小，而且在形態上種間常有覆蓋，而種內有較大的變異，僅依據形態特徵很難鑒定。分子鑒定技術給植物線蟲的檢測和鑒定提供瞭快速、精確、可靠的方法。文章綜述瞭植物線蟲分子的 DNA 提取、分子鑒定靶標序列的選擇及分子鑒定方法等方麵的研究進展及現狀，以促進對植物線蟲分子鑒定更深入的研究。
식물선충가위해농작물화림목，대타문적준학감정시방치식물선충병해적기출。유우식물선충흔소，이차재형태상충간상유복개，이충내유교대적변이，부의거형태특정흔난감정。분자감정기술급식물선충적검측화감정제공료쾌속、정학、가고적방법。문장종술료식물선충분자적 DNA 제취、분자감정파표서렬적선택급분자감정방법등방면적연구진전급현상，이촉진대식물선충분자감정경심입적연구。
Plant nematodes can damage crops and trees.The accurate identification of plant nematodes is the basis for the control of plant nematode diseases.It is very challenging to identify plant nematode species only based on morphology,because plant nematodes are microscopic in size,and some morphological characters may have inter-specific overlapping or intraspecific variability.Molecular techniques provide a fast,accurate and reliable method for the detection and identification of plant nematodes.For promoting the deep study on molecular identification of plant nematodes,this paper summarized research progresses in and current research status on DNA extraction of plant nematodes,target sequences for identifying plant nematodes and the main molecular identification meth-ods for plant nematodes.
