CAJ | 학술논문

采集病死鸡法氏囊经处理后绒毛尿囊膜途径接种5枚SPF鸡胚，接种胚均于接种后48~96 h死亡，死亡胚病变特征为体表和皮下出血，胚水肿，肾脏出血，肝脏有坏死灶，心肌苍白。琼扩试验结果显示法氏囊样品孔、绒毛尿囊膜样品孔与标准血清孔间均可出现明显的沉淀线，说明样品中含有传染性法氏囊病病毒。根据实验室检查情况，诊断为鸡传染性法氏囊病。
채집병사계법씨낭경처리후융모뇨낭막도경접충5매SPF계배，접충배균우접충후48~96 h사망，사망배병변특정위체표화피하출혈，배수종，신장출혈，간장유배사조，심기창백。경확시험결과현시법씨낭양품공、융모뇨낭막양품공여표준혈청공간균가출현명현적침정선，설명양품중함유전염성법씨낭병병독。근거실험실검사정황，진단위계전염성법씨낭병。
Suspensions of tissues (20% w/v) were prepared in sterile phosphate buffered saline (PBS) added with sutiable antibiotics. The suspensions were clarified by low-speed centrifugation and 0.2 mL samples were inoculated into the chorioallantoic membrane of 10-day-old embryos. Eggs were candled daily for 5 days with mortality within the first 24 hours being considered nonspecific. Death of embryos occurred between 48~96 h post inoculation and showed specific features of IBDV infection . The samples of bursa collected from dead chicks and chorioallantoic membrane harvested from dead embryos were prepared for Agar gel immunodiffusion test. The results showed that there were clear white sediment lines between samples and standard antisera. According to the above results, the case could be determined as Infectious bursal disease.