浙江大学学报(农业与生命科学版)
浙江大學學報(農業與生命科學版)
절강대학학보(농업여생명과학판)
Journal of Zhejiang University (Agriculture & Life Sciences)
2015年
6期
712-722
,共11页
王青松%柳永%王新%孙宏%姚晓红%吴逸飞%汤江武%葛向阳
王青鬆%柳永%王新%孫宏%姚曉紅%吳逸飛%湯江武%葛嚮暘
왕청송%류영%왕신%손굉%요효홍%오일비%탕강무%갈향양
载菌胶囊%质构量化分析%内部结构%混合比例%氨氮降解
載菌膠囊%質構量化分析%內部結構%混閤比例%氨氮降解
재균효낭%질구양화분석%내부결구%혼합비례%안담강해
bacteria-embedded capsules%texture profile analysis%internal structure%blending ratio%ammonia-nitrogen degradation
为提高净水菌剂的应用性能,通过固定化包埋处理,以实心载菌胶囊为研究模型,以质构量化分析数据为主要筛选依据,通过不同辅料配方和制作工艺对胶囊的硬度、黏聚性和弹性恢复进行比较研究,以筛选出较佳的辅料配方和加工工艺,并对较佳配方的载菌胶囊进行断面形貌观察、孔隙率测定、菌剂释放率测定和氨氮模拟污水净化实验.结果表明:较佳成囊壁材配方为V (4%海藻酸钠)∶V (10%聚乙烯醇)=1∶9,固化液配方为4%H3 BO4和4% CaCl2混合溶液,辅料硅藻土、沸石粉和竹炭的适宜添加量分别为20%~30%、20%~80%和20%~60%;胶囊为多孔结构,单独添加硅藻土、沸石粉、竹炭或三者混合添加下,胶囊微观结构存在差异,其中硅藻土、沸石粉和竹炭经包埋后比表面积和孔容均减小,孔径变化不大;24 h内胶囊中菌剂的水体释放率较低,为1%~14%;载菌胶囊在模拟污水净化实验中,20 h后各实验组氨氮质量浓度从1 h时的39.3~44.7 m g/L降低到0.1 mg/L 以下,其中未经包埋的菌株直接投放组降低最快,其次为混合1组[ V (4%海藻酸钠)∶V (10%聚乙烯醇)=1∶9,海藻酸钠和聚乙烯醇总体积10mL+硅藻土1.5g+沸石粉0.5g+竹炭0.5g+菌悬液2 mL ]和竹炭组,同时,总氮也出现不同程度的降低.总之,该研究采用的质构量化分析方法为净水菌固定化载体研究提供了可靠的数据支撑,研究获得的优化胶囊体系在质构和净水能力方面均表现出良好的性能,在环境污染水体微生物净化治理方面具有较大的应用潜力.
為提高淨水菌劑的應用性能,通過固定化包埋處理,以實心載菌膠囊為研究模型,以質構量化分析數據為主要篩選依據,通過不同輔料配方和製作工藝對膠囊的硬度、黏聚性和彈性恢複進行比較研究,以篩選齣較佳的輔料配方和加工工藝,併對較佳配方的載菌膠囊進行斷麵形貌觀察、孔隙率測定、菌劑釋放率測定和氨氮模擬汙水淨化實驗.結果錶明:較佳成囊壁材配方為V (4%海藻痠鈉)∶V (10%聚乙烯醇)=1∶9,固化液配方為4%H3 BO4和4% CaCl2混閤溶液,輔料硅藻土、沸石粉和竹炭的適宜添加量分彆為20%~30%、20%~80%和20%~60%;膠囊為多孔結構,單獨添加硅藻土、沸石粉、竹炭或三者混閤添加下,膠囊微觀結構存在差異,其中硅藻土、沸石粉和竹炭經包埋後比錶麵積和孔容均減小,孔徑變化不大;24 h內膠囊中菌劑的水體釋放率較低,為1%~14%;載菌膠囊在模擬汙水淨化實驗中,20 h後各實驗組氨氮質量濃度從1 h時的39.3~44.7 m g/L降低到0.1 mg/L 以下,其中未經包埋的菌株直接投放組降低最快,其次為混閤1組[ V (4%海藻痠鈉)∶V (10%聚乙烯醇)=1∶9,海藻痠鈉和聚乙烯醇總體積10mL+硅藻土1.5g+沸石粉0.5g+竹炭0.5g+菌懸液2 mL ]和竹炭組,同時,總氮也齣現不同程度的降低.總之,該研究採用的質構量化分析方法為淨水菌固定化載體研究提供瞭可靠的數據支撐,研究穫得的優化膠囊體繫在質構和淨水能力方麵均錶現齣良好的性能,在環境汙染水體微生物淨化治理方麵具有較大的應用潛力.
위제고정수균제적응용성능,통과고정화포매처리,이실심재균효낭위연구모형,이질구양화분석수거위주요사선의거,통과불동보료배방화제작공예대효낭적경도、점취성화탄성회복진행비교연구,이사선출교가적보료배방화가공공예,병대교가배방적재균효낭진행단면형모관찰、공극솔측정、균제석방솔측정화안담모의오수정화실험.결과표명:교가성낭벽재배방위V (4%해조산납)∶V (10%취을희순)=1∶9,고화액배방위4%H3 BO4화4% CaCl2혼합용액,보료규조토、비석분화죽탄적괄의첨가량분별위20%~30%、20%~80%화20%~60%;효낭위다공결구,단독첨가규조토、비석분、죽탄혹삼자혼합첨가하,효낭미관결구존재차이,기중규조토、비석분화죽탄경포매후비표면적화공용균감소,공경변화불대;24 h내효낭중균제적수체석방솔교저,위1%~14%;재균효낭재모의오수정화실험중,20 h후각실험조안담질량농도종1 h시적39.3~44.7 m g/L강저도0.1 mg/L 이하,기중미경포매적균주직접투방조강저최쾌,기차위혼합1조[ V (4%해조산납)∶V (10%취을희순)=1∶9,해조산납화취을희순총체적10mL+규조토1.5g+비석분0.5g+죽탄0.5g+균현액2 mL ]화죽탄조,동시,총담야출현불동정도적강저.총지,해연구채용적질구양화분석방법위정수균고정화재체연구제공료가고적수거지탱,연구획득적우화효낭체계재질구화정수능력방면균표현출량호적성능,재배경오염수체미생물정화치리방면구유교대적응용잠력.
Summary The technology for microorganism immobilization originated from the immobilized enzyme technology in the 1970s . After decades of development , it has been widely used in food fermentation and environmental protection , etc . Especially , microbe‐embedded capsules have drawn substantial attentions from researchers due to their significant roles in water purification and sewage treatment . However , lack of consolidated standards has impeded their applications . Texture profile analysis ( TPA) has been widely used in food industry for determination of product structure and quality , while its application in non‐food field is yet to be explored . Therefore , in the present study , TPA was used for the development and optimization of bacteria‐embedded solid capsule systems in the purpose of water purification . To achieve this purpose , solid capsules were prepared with different materials , such as diatomite , zeolite powder and bamboo charcoal in different blending ratios . TPA was employed to characterize these capsules in respects of hardness , cohesiveness and elastic resilience ( springiness) . Meanwhile , scanning electron microscopy ( SEM ) was adopted to investigate the sectional structure and pore size of these capsules . Furthermore , simulative experiments in both ultrapure water and sewage were carried out to examine the releasing velocity and water purification efficiency of these bacteria‐embedded solid capsules . Combining with TPA and SEM , the factors influencing the internal structure of capsules including embedding/crosslinking medium , curing duration , and ingredient proportions were explored , thus providing standards and guidelines for the preparation of bacteria‐embedded capsules for large‐scale practical applications in the future . The TPA data showed that a high ratio of sodium alginate in the embedding medium resulted in capsules with high strength , and polyvinyl alcohol rendered cohesiveness and resilience ( springiness) to capsules . An optimal embedding medium system was established as follows: V (4% sodium alginate) ∶ V (10% polyvinyl alcohol) =1∶9 for embedding medium , while the mixture of 4% boric acid and 4% calcium chloride for crosslinking medium . The optimal concentrations of various materials were determined as follows: 20% 30% for diatomite , 20% 80% for zeolite powder and 20% 60% for bamboo charcoal . The optimal curing duration varied from 16 to 36 h . The SEM images indicated the existence of internal microspores both at nanoscale and micron‐size in the crosslinked capsules , and differences on microstructures of capsules were observed among single or combining addition of diatomite , zeolite powder and bamboo charcoal . Both specific surface area and pore volume decreased after embedding , but not for pore size . The releasing rates of bacteria in capsules in 24 h were low , from 1% to 14% . In the simulative experiments of the sewage purification , all groups , including bacteria‐embedded capsule and free bacteria , showed good ammonia removal efficiency . The final concentration of ammonia was below 0 .1 mg/L after 20 h , while the initial concentration was 39 .3 44 .7 mg/L . The bacteria‐free group had the highest ammonia removal efficiency , followed by mixture 1 group (1 mL of 4% sodium alginate , 9 mL of 10% polyvinyl alcohol , 1 .5 g diatomite , 0 .5 g zeolite powder , 0 .5 g bamboo charcoal , and 2 mL bacterial culture) and bamboo charcoal group . Meanwhile , the total nitrogen concentration decreased at a certain degree . These results confirmed that these capsules were applicable for sewage treatment . In conclusion , the present study establishes successfully standards and guidelines for the preparation of bacteria‐embedded capsules on the basis of TPA in combination with SEM and simulative experiments . This profound step can accelerate the practical application of bacteria‐embedded capsules in actual sewage purification .