CAJ | 학술논문

对绿豆叶斑病菌进行形态学和分子特征鉴定,结果表明,分离物在 V8培养基上菌落近圆形,菌落边缘泛紫色,分生孢子无色；用通用引物18SF 和28SR 扩增6个分离物的 rDNA-ITS 部分序列和测序,并到 GenBank (登录号：KM435076)数据库进行比对,6个分离物与变灰尾孢分离物序列相似性为99%,基于形态和分子特征将绿豆叶斑病病原菌鉴定为变灰尾孢。致病性测定结果表明,6个分离物对绿豆‘中绿一号’叶片均致病且存在致病力差异。不同培养基、温度和 pH 对病原菌生长的影响研究表明,V8培养基最适宜该病菌生长,病菌生长的最适温度为25℃,最适 pH 为6。
대록두협반병균진행형태학화분자특정감정,결과표명,분리물재 V8배양기상균락근원형,균락변연범자색,분생포자무색；용통용인물18SF 화28SR 확증6개분리물적 rDNA-ITS 부분서렬화측서,병도 GenBank (등록호：KM435076)수거고진행비대,6개분리물여변회미포분리물서렬상사성위99%,기우형태화분자특정장록두협반병병원균감정위변회미포。치병성측정결과표명,6개분리물대록두‘중록일호’협편균치병차존재치병력차이。불동배양기、온도화 pH 대병원균생장적영향연구표명,V8배양기최괄의해병균생장,병균생장적최괄온도위25℃,최괄 pH 위6。
The morphology and molecular characteristics of mung bean leaf spot were studied.The results indica-ted that the colony of isolates was circular in V8 medium,the edge was purple and the conidia were colorless.The partial rDNA-ITS sequences of six isolates were amplified with universal primers 18SF and 28SR.The obtained ITS sequences (GenBank accession No.KM435076)were aligned with GenBank and showed 99% similarity with iso-lates of Cercospora canescens .Based on morphological and molecular characteristics,the pathogen of mung bean leaf spot was identified as C .canescens .Pathogenicity test results showed that all mung bean cv ‘Zhonglv No.1’ leaves showed dark brown lesions when inoculated with six isolates and had virulence differences.The influences of different media,temperatures and pH values on the growth of pathogen were observed.V8 was the optimum culture medium for pathogen growth,at 25 ℃ and pH 6.