CAJ | 학술논문

目的：建立保健品中非法掺入硫代伐地那非的液相色谱-质谱联用法(HPLC-MS)检测方法。方法保健品样品经甲醇超声提取得提取液,用液相色谱-串联四极杆质谱进行分析,根据相对分子量、液相色谱保留时间、色谱峰面积等,对保健品中非法掺入的硫代伐地那非进行定性以及定量分析。结果在10~200μg/mL浓度范围内,硫代伐地那非浓度与峰面积呈良好线性关系, r2=0.998,精密度和稳定性良好,加样平均回收率为99.9%, RSD为0.613%,方法准确可靠,专属性强,并测得某市售保健品中硫代伐地那非的含量为0.34%。结论液相色谱-质谱联用法专属性强,灵敏度高,可作为检测保健品中非法掺入硫代伐地那非的有效方法。
목적：건립보건품중비법참입류대벌지나비적액상색보-질보련용법(HPLC-MS)검측방법。방법보건품양품경갑순초성제취득제취액,용액상색보-천련사겁간질보진행분석,근거상대분자량、액상색보보류시간、색보봉면적등,대보건품중비법참입적류대벌지나비진행정성이급정량분석。결과재10~200μg/mL농도범위내,류대벌지나비농도여봉면적정량호선성관계, r2=0.998,정밀도화은정성량호,가양평균회수솔위99.9%, RSD위0.613%,방법준학가고,전속성강,병측득모시수보건품중류대벌지나비적함량위0.34%。결론액상색보-질보련용법전속성강,령민도고,가작위검측보건품중비법참입류대벌지나비적유효방법。
ABSTRACT:Objective To develop a high performance liquid chromatography-mass spectrometry (HPLC-MS) method for the identification of illegally added thiovardenafil in healthcare foods.Methods Thiovardenafil in healthcare food was extracted with methyl and then analyzed by HPLC-MS. Qualitative and quantitative analysis were conducted according to relative molecular weight, retention time and peak aera.Re-sultsGood linearly correlation were detected in the range of 10 to 200μg/mL (r2=0.998, n=5). The average recovery rate was 99.9%, and RSD was 0.613% (n=6). The content of thiovardenafil the in a market sample was 0.34%.ConclusionThe method is selective and sensitive, which can be used to detect thiovardenafil il-legally added in healthcare food suplements.