CAJ | 학술논문

经形态学特征观察、致病性测定及 rDNA-ITS 序列分析，首次确定红豆杉根腐病病原为尖孢镰刀菌(Fusari-um oxysporum )。采用平板对峙培养法从健康植株根际土壤中筛选得到3株对尖孢镰刀菌具有明显拮抗作用的芽胞杆菌 YB6、YB15、YB37。经形态学、生理生化及16S rDNA 同源性分析，并通过构建16S rDNA 系统发育树，将YB6、YB15和 YB37分别鉴定为蜡样芽胞杆菌(Bacillus cereus )、解淀粉芽胞杆菌(B .amyloliquefaciens )和多黏类芽胞杆菌(Paenibacillu polymyxa )，其中多黏类芽胞杆菌拮抗效果最好，具有进一步研究开发的潜力。本研究通过对南方红豆杉根腐病病原鉴定及其拮抗芽胞杆菌的筛选，对该病的防治提供了理论依据。
경형태학특정관찰、치병성측정급 rDNA-ITS 서렬분석，수차학정홍두삼근부병병원위첨포렴도균(Fusari-um oxysporum )。채용평판대치배양법종건강식주근제토양중사선득도3주대첨포렴도균구유명현길항작용적아포간균 YB6、YB15、YB37。경형태학、생리생화급16S rDNA 동원성분석，병통과구건16S rDNA 계통발육수，장YB6、YB15화 YB37분별감정위사양아포간균(Bacillus cereus )、해정분아포간균(B .amyloliquefaciens )화다점류아포간균(Paenibacillu polymyxa )，기중다점류아포간균길항효과최호，구유진일보연구개발적잠력。본연구통과대남방홍두삼근부병병원감정급기길항아포간균적사선，대해병적방치제공료이론의거。
In this study,the pathogen isolated from root rot of Taxus chinensis var.mairer was first indentified. Based on morphological characteristics,pathogenicity and rDNA ITS sequence analysis,the results showed that the pathogen of root-rot disease of T .chinensis var.mairer was Fusarium oxysporum .The tablet confrontation training method was adopted to screen the antagonistic Bacillus species from the healthy plant rhizosphere,and three strains with obvious antagonistic effect were obtained,namely YB6,YB1 5 and YB37.Through morphologi-cal,physiological,biochemical and 1 6S rDNA homology analysis as well as the construction of 1 6S rDNA phy-logenetic tree,we found that YB6,YB15 and YB37 were Bacillus cereus ,B .amyloliquefaciens and Paenibacillus poly-myxa ,respectively.The antagonistic effect of P .polymyxa was the best with further research and development potential. This study provides some valuable information for effective prevention and treatment of this disease.