CAJ | 학술논문

目的：研究制备适用于菌落总数能力验证冷冻干燥样品的方法，并对样品的稳定性和均匀性进行评估。方法采用真空冷冻干燥方式分别制备菌落总数能力验证阳性样品与阴性样品，西林瓶真空包装、4℃冷藏贮存，制备出以脱脂奶粉为基质的均匀性好、稳定性强的菌落总数能力验证样品。通过随机抽样以评估样品的均匀性；通过观察样品在12 w内不同温度下菌落总数的测试结果以评估样品的稳定性。结果随机抽检的样品具有较好的均匀性；贮存温度对样品稳定性有较大影响，高温明显降低样品稳定性；通过保存温度和时间的稳定性检验显示测试结果符合预期效果，样品具有较好的稳定性。结论建立了有效的菌落总数能力验证样品制备的方法与评价程序。
목적：연구제비괄용우균락총수능력험증냉동간조양품적방법，병대양품적은정성화균균성진행평고。방법채용진공냉동간조방식분별제비균락총수능력험증양성양품여음성양품，서림병진공포장、4℃랭장저존，제비출이탈지내분위기질적균균성호、은정성강적균락총수능력험증양품。통과수궤추양이평고양품적균균성；통과관찰양품재12 w내불동온도하균락총수적측시결과이평고양품적은정성。결과수궤추검적양품구유교호적균균성；저존온도대양품은정성유교대영향，고온명현강저양품은정성；통과보존온도화시간적은정성검험현시측시결과부합예기효과，양품구유교호적은정성。결론건립료유효적균락총수능력험증양품제비적방법여평개정서。
ABSTRACT:Objective The preparation of lyophilized samples were applied in bacterial countproficiency testing, to assess the homogeneity and stability of samples.Method Bacterial count proficiency testing pos-itive samples and negative samples were made by vacuum freeze drying method in penicillin bottles vacuum packaging and stored at 4℃ . The uniformity of the proficiency testing samples was evaluated by random sam-pling, and the stability of proficiency testing samples was kept for 12 weeks.Results Random samples had a preferable accordance ratio, and could keep stable for at least 12 weeks while stored at different temperatures. Suitable bacterial count proficiency testing samples and effective sample preparation and evaluation process were established. Conclusion Effective bacterial count proficiency testing sample preparation method and evaluation process were established.