CAJ | 학술논문

目的：验证呋喃妥因代谢物CLIA检测试剂盒的检测效果。方法用化学发光微粒子免疫法和高效液相色谱-串联质谱法分别对市售猪肉、鸡肉、鱼肉、虾肉4种样品中呋喃妥因代谢物残留量进行检测,比对两种方法试验结果间的差异。结果该试剂盒对4种样品的最低检测限分别为69.37、67.86、67.52、69.09 ng/kg,试剂盒板内板间变异系数均小于10%；对4种样品做加标回收试验,其回收率均在90%~105%之间,变异系数均小于10%；该方法与高效液相色谱法检测实际样品的阴、阳性判断结果一致。结论该方法稳定、可靠,可满足食品中呋喃妥因代谢物残留快速检测的需要。
목적：험증부남타인대사물CLIA검측시제합적검측효과。방법용화학발광미입자면역법화고효액상색보-천련질보법분별대시수저육、계육、어육、하육4충양품중부남타인대사물잔류량진행검측,비대량충방법시험결과간적차이。결과해시제합대4충양품적최저검측한분별위69.37、67.86、67.52、69.09 ng/kg,시제합판내판간변이계수균소우10%；대4충양품주가표회수시험,기회수솔균재90%~105%지간,변이계수균소우10%；해방법여고효액상색보법검측실제양품적음、양성판단결과일치。결론해방법은정、가고,가만족식품중부남타인대사물잔류쾌속검측적수요。
ABSTRACT:Objective To verify the test effect of chemiluminescence immune (CLIA) kit. Methods The method of CLIA was used to detect the residues of furantoin metabolites in 4 kinds of samples including market sold pork, chicken, fish and shrimp, and the results were compared with that of high performance liquid chro-matography-tandem mass spectrometry.Results The minimum detection limitation of the kit of the 4 kinds of samples were 69.37, 67.86, 67.52, and 69.09 ng/kg, respectively, and the coefficient of variation was less than 10%.The recovery of the method was in the range of 90%~105%, and the coefficient of variation was less than 10%. The CLIA's judgments of positive and negative were the same as the HPLC's.Conclusion This method is efficient, reliable and sensitive, and can meet the fast-tested need for the determination of furantoin metabo-lites residues in food.