CAJ | 학술논문

土壤强还原(reductive soil disinfestation,RSD)能有效杀灭土传病原菌,并已在日本、荷兰和美国取得一系列的推广应用,但对于 RSD 的杀菌机理不甚明了。本试验采用高效液相色谱和荧光定量 PCR 等技术研究了以玉米秸秆为有机碳源的 RSD 处理过程中土壤产生的有机酸种类,以及不同浓度的有机酸对土壤病原菌的抑制效果。结果表明：以玉米秸秆为有机碳源的 RSD 过程中主要产生了乙酸和丁酸；经50 mmol/L 丁酸溶液处理的土壤中立枯丝核菌、辣椒疫霉、尖孢镰刀菌及茄劳尔氏菌的数量分别为对照的3.5%、38.9%、11.5%和7.9%；10 mmol/L 的丁酸可以完全抑制尖孢镰刀菌菌丝的生长；5 mmol/L 的丁酸即可完全抑制尖孢镰刀菌的孢子萌发。本研究结合前人报道表明有机酸,尤其是乙酸和丁酸,对于 RSD 的杀菌机理起着重要的作用。
토양강환원(reductive soil disinfestation,RSD)능유효살멸토전병원균,병이재일본、하란화미국취득일계렬적추엄응용,단대우 RSD 적살균궤리불심명료。본시험채용고효액상색보화형광정량 PCR 등기술연구료이옥미갈간위유궤탄원적 RSD 처리과정중토양산생적유궤산충류,이급불동농도적유궤산대토양병원균적억제효과。결과표명：이옥미갈간위유궤탄원적 RSD 과정중주요산생료을산화정산；경50 mmol/L 정산용액처리적토양중립고사핵균、랄초역매、첨포렴도균급가로이씨균적수량분별위대조적3.5%、38.9%、11.5%화7.9%；10 mmol/L 적정산가이완전억제첨포렴도균균사적생장；5 mmol/L 적정산즉가완전억제첨포렴도균적포자맹발。본연구결합전인보도표명유궤산,우기시을산화정산,대우 RSD 적살균궤리기착중요적작용。
Reductive soil disinfestation (RSD)is an effective way to suppress soil-borne plant pathogens.Although it is increasingly used in USA,Netherlands and Japan,its precise mechanism has not been well elucidated so far. High performance liquid chromatography and quantitative real-time PCR were used for investigating the organic acids produced in maize straw amended RSD and the inhibitory effect of organic acids on the soilborne pathogens in this work.The results showed that acetic acid and butyric acid were the primary compounds produced in maize straw amended RSD.The populations of Rhizoctonia solani ,Phytophthora capsici ,Fusarium oxysporum and Ral-stonia solanacearum in the diseased soil treated by 50 mmol/L butyric acid solution decreased by 96.5%,61.1%, 88.5% and 82.1%,respectively,compared with those in non-treated diseased soil.The growth of F .oxysporum mycelium was completely stopped in 1/4 potato dextrose agar medium containing 5 mmol/L butyric acid,and 5 mmol/L butyric acid could entirely inhibit the germination of F .oxysporum spores.This work together with previous reports indicated that organic acids,especially acetic and butyric acid,played an important role in the mechanism of RSD.