CAJ | 학술논문

目的：利用连续流动分析仪建立以水杨酸-次氯酸盐比色法为基础的葡萄酒和葡萄汁中氨氮的分析方法。方法葡萄汁及葡萄酒样品经过滤后直接进样,试样与试剂在蠕动泵的推动下进入化学反应模块,在密闭的管路中连续流动,并按特定的顺序和比例混合、反应,显色完全后进入流动检测池进行光度检测。结果氨氮标准溶液利用12%乙醇溶液配制线性范围为0~60 mg/L,相关系数(r2)大于0.999；方法检出限(LOD)为0.015 mg/L。在低、中、高三个浓度水平添加下,样品氨氮回收率范围为90.0%~96.8%,相对标准偏差(n=6)均小于1%。结论该方法灵敏度高,重复性好,操作简便,适用于大批量葡萄酒和葡萄汁中氨氮含量的检测。
목적：이용련속류동분석의건립이수양산-차록산염비색법위기출적포도주화포도즙중안담적분석방법。방법포도즙급포도주양품경과려후직접진양,시양여시제재연동빙적추동하진입화학반응모괴,재밀폐적관로중련속류동,병안특정적순서화비례혼합、반응,현색완전후진입류동검측지진행광도검측。결과안담표준용액이용12%을순용액배제선성범위위0~60 mg/L,상관계수(r2)대우0.999；방법검출한(LOD)위0.015 mg/L。재저、중、고삼개농도수평첨가하,양품안담회수솔범위위90.0%~96.8%,상대표준편차(n=6)균소우1%。결론해방법령민도고,중복성호,조작간편,괄용우대비량포도주화포도즙중안담함량적검측。
ABSTRACT:Objective To establish a method for the determination of ammonium nitrogen in wines and grape juice by continuous flow analysis (CFA) and salicylate spectrophotometry.Methods The automatic dosage of wines and grape juice were introduced into a flow system (manifold) in which the samples mixed, reacted with the reagent solutions on their way through the manifold by pump. The reaction product was meas-ured in a flow detector.Results The perfect linearity (r2>0.999) of ammonium nitrogen standards, which were prepared with 12% ethnol-water, was achieved within the ranges of 0~60 mg/L. The detection limit of the method was 0.015 mg/L. The recoveries of ammonium at 3 different spiked levels (low, medium, high) were within range from 90.0% to 96.8% and the relative standard deviations (RSDs,n=6) were below 1%.Conclusion This me-thod is efficient, sensitive, reproducible, and reliable, and suitable for the determination of ammonia nitrogen in large number of samples of wines and grape juice.