通过造小鼠糖尿病模型并给予不同剂量的雨生红球藻提取物,研究其对糖尿病造模小鼠糖耐量和胰岛素耐量的影响、血脂的调节情况及改善糖尿病病情的可能的作用机制.通过四氧嘧啶腹腔注射的方式建立糖尿病小鼠模型,灌胃给予不同剂量的雨生红球藻提取物,给药期为28d,每7d记录1次小鼠空腹血糖及体质量；给药期末进行耐糖量和胰岛素耐量实验,并测定血清甘油三酯( triglyceride ,TG)、血清总胆固醇( serum total cholesterol ,TC)、高密度脂蛋白胆固醇( high density lipoprotein cholesterol ,HDL‐C)和低密度脂蛋白胆固醇( low density lipoprotein cholesterol ,LDL‐C)含量.结果表明：造模后小鼠毛色杂乱暗淡,出现明显多饮、多食、多尿的三多"症状,小鼠体质量明显减轻.试验组给予不同剂量雨生红球藻提取物28 d后,高、中、低剂量组的体质量与模型对照组比较均有统计学上的显著差异( P＜0.01,P＜0.05,P＜0.05),高、中剂量组的血糖与模型组比较均有统计学上的显著差异(P＜0.05,P＜0.05),且以高剂量组最为明显.在糖耐量实验中,各组小鼠的血糖值均在糖负荷30 min时达到峰值,30 min后血糖值随着时间的增加呈下降趋势；高剂量组和中剂量组血糖值在120 min时明显低于模型对照组( P＜0.01).在胰岛素耐量实验中,腹腔注射0.8 U/kg胰岛素15 min后,高剂量组血糖值较基础值降低20.9％,而低剂量组仅降低0.6％；3个剂量组在90 min时均降低至最低值,血糖分别降低53.6％、23.9％和17.9％,模型组血糖降低5.3％；120 min时血糖已上升,但雨生红球藻提取物高剂量组血糖值仍显著低于模型对照组( P＜0.01).雨生红球藻提取物高剂量组血清TC、TG及LDL‐C含量明显低于模型对照组,差异具有统计学意义(P＜0.05,P＜0.01,P＜0.05).总之,雨生红球藻提取物可显著降低造模糖尿病小鼠的血糖水平,缓解多饮、多食、多尿及体质量减轻症状,显著改善病鼠机体的糖耐量及胰岛素耐量,提升其对葡萄糖的利用能力,有效调节病鼠的血脂紊乱,改善血液环境,且对正常小鼠的血糖、血脂无显著影响.在实验所用的3个剂量中,以高剂量(8 g/kg)效果最佳.
通過造小鼠糖尿病模型併給予不同劑量的雨生紅毬藻提取物,研究其對糖尿病造模小鼠糖耐量和胰島素耐量的影響、血脂的調節情況及改善糖尿病病情的可能的作用機製.通過四氧嘧啶腹腔註射的方式建立糖尿病小鼠模型,灌胃給予不同劑量的雨生紅毬藻提取物,給藥期為28d,每7d記錄1次小鼠空腹血糖及體質量；給藥期末進行耐糖量和胰島素耐量實驗,併測定血清甘油三酯( triglyceride ,TG)、血清總膽固醇( serum total cholesterol ,TC)、高密度脂蛋白膽固醇( high density lipoprotein cholesterol ,HDL‐C)和低密度脂蛋白膽固醇( low density lipoprotein cholesterol ,LDL‐C)含量.結果錶明：造模後小鼠毛色雜亂暗淡,齣現明顯多飲、多食、多尿的三多"癥狀,小鼠體質量明顯減輕.試驗組給予不同劑量雨生紅毬藻提取物28 d後,高、中、低劑量組的體質量與模型對照組比較均有統計學上的顯著差異( P＜0.01,P＜0.05,P＜0.05),高、中劑量組的血糖與模型組比較均有統計學上的顯著差異(P＜0.05,P＜0.05),且以高劑量組最為明顯.在糖耐量實驗中,各組小鼠的血糖值均在糖負荷30 min時達到峰值,30 min後血糖值隨著時間的增加呈下降趨勢；高劑量組和中劑量組血糖值在120 min時明顯低于模型對照組( P＜0.01).在胰島素耐量實驗中,腹腔註射0.8 U/kg胰島素15 min後,高劑量組血糖值較基礎值降低20.9％,而低劑量組僅降低0.6％；3箇劑量組在90 min時均降低至最低值,血糖分彆降低53.6％、23.9％和17.9％,模型組血糖降低5.3％；120 min時血糖已上升,但雨生紅毬藻提取物高劑量組血糖值仍顯著低于模型對照組( P＜0.01).雨生紅毬藻提取物高劑量組血清TC、TG及LDL‐C含量明顯低于模型對照組,差異具有統計學意義(P＜0.05,P＜0.01,P＜0.05).總之,雨生紅毬藻提取物可顯著降低造模糖尿病小鼠的血糖水平,緩解多飲、多食、多尿及體質量減輕癥狀,顯著改善病鼠機體的糖耐量及胰島素耐量,提升其對葡萄糖的利用能力,有效調節病鼠的血脂紊亂,改善血液環境,且對正常小鼠的血糖、血脂無顯著影響.在實驗所用的3箇劑量中,以高劑量(8 g/kg)效果最佳.
통과조소서당뇨병모형병급여불동제량적우생홍구조제취물,연구기대당뇨병조모소서당내량화이도소내량적영향、혈지적조절정황급개선당뇨병병정적가능적작용궤제.통과사양밀정복강주사적방식건립당뇨병소서모형,관위급여불동제량적우생홍구조제취물,급약기위28d,매7d기록1차소서공복혈당급체질량；급약기말진행내당량화이도소내량실험,병측정혈청감유삼지( triglyceride ,TG)、혈청총담고순( serum total cholesterol ,TC)、고밀도지단백담고순( high density lipoprotein cholesterol ,HDL‐C)화저밀도지단백담고순( low density lipoprotein cholesterol ,LDL‐C)함량.결과표명：조모후소서모색잡란암담,출현명현다음、다식、다뇨적삼다"증상,소서체질량명현감경.시험조급여불동제량우생홍구조제취물28 d후,고、중、저제량조적체질량여모형대조조비교균유통계학상적현저차이( P＜0.01,P＜0.05,P＜0.05),고、중제량조적혈당여모형조비교균유통계학상적현저차이(P＜0.05,P＜0.05),차이고제량조최위명현.재당내량실험중,각조소서적혈당치균재당부하30 min시체도봉치,30 min후혈당치수착시간적증가정하강추세；고제량조화중제량조혈당치재120 min시명현저우모형대조조( P＜0.01).재이도소내량실험중,복강주사0.8 U/kg이도소15 min후,고제량조혈당치교기출치강저20.9％,이저제량조부강저0.6％；3개제량조재90 min시균강저지최저치,혈당분별강저53.6％、23.9％화17.9％,모형조혈당강저5.3％；120 min시혈당이상승,단우생홍구조제취물고제량조혈당치잉현저저우모형대조조( P＜0.01).우생홍구조제취물고제량조혈청TC、TG급LDL‐C함량명현저우모형대조조,차이구유통계학의의(P＜0.05,P＜0.01,P＜0.05).총지,우생홍구조제취물가현저강저조모당뇨병소서적혈당수평,완해다음、다식、다뇨급체질량감경증상,현저개선병서궤체적당내량급이도소내량,제승기대포도당적이용능력,유효조절병서적혈지문란,개선혈액배경,차대정상소서적혈당、혈지무현저영향.재실험소용적3개제량중,이고제량(8 g/kg)효과최가.
Summary Diabetes was generally recognized as a critical issue in global public health , and has become the third noninfectious disease after angiocardiopathy and cancer . So far , the combination of applying insulin and controlling diet , as well as changing the way of living were the major therapeutic method for treating diabetes . Recently , the adjuvant therapy of natural extracts has been the research highlight in this field . To observe the intervention effect of Haematococcus pluvialis extract on blood glucose tolerance , insulin tolerance , lipid adjustment and the possible functional mechanism of improving diabetic patients condition , the diabetic mouse models were induced by alloxan through intraperitoneal injection , and given by gavage with different doses of H . pluvialis extract for 28 days . The contents of fasting blood glucose and their body masses were detected at 7‐day intervals . The capability of blood glucose tolerance and insulin tolerance was measured during later period of administration . Meanwhile , the contents of triacylglycerol ( TG) , serum total cholesterol ( TC) , high density lipoprotein cholesterol ( HDL‐C ) and low density lipoprotein cholesterol ( LDL‐C ) were detected . After modeling , the mice had dark and messy hair , and the typical diabetic symptoms including polydipsia , polyphagia and polyuria along with significant mass loss . Compared with the model group , by giving different doses of H . pluvialis extract in 28 d for experimental groups , the high , middle and low dose groups had statistically significant differences between the body masses and blood glucose contents , respectively ( P<0 .01 , P<0 .05 , P<0 .05) . The difference between high dose group and model group was most evident . During the experiment of blood glucose tolerance , the blood glucose level of each group reached the peak in 30 min of glucose loading . The glucose levels appeared downtrend after 30 min ,of which the high dose group and middle dose group were much lower than the model group in 120 min ( P<0 .01) . In the insulin experiment , after injecting 0 .8 U/kg insulin for 15 min , the blood glucose level of high dose group reduced by 20 .9% compared with the basic level , while the low dose group only reduced by 0 .6% . Three dose groups had the lowest blood glucose levels which reduced by 53 .6% , 23 .9% and 17 .9% respectively in 90 min , while the model group reduced by 5 .3% . The blood glucose increased in 120 min;however , the level in the high dose group of H . pluvialis extract was still much lower than that of model group ( P<0 .01) . The TC , TG and LDL‐C contents of the high dose group of H . pluvialis extract were much lower than the model group , which were statistically significant differences ( P<0.05,P<0.01,P<0.05) . Haematococcus pluvialis extract has remarkable effect of decreasing blood glucose level , relieving the symptoms of polydipsia , polyphagia , polyuria and mass loss , improving the glucose tolerance and insulin tolerance , increasing the utilization of glucose and regulating blood lipid level on diabetic mice . However , it has no effect on the blood glucose and lipids of normal mice . Among the three dose groups , the high dose group ( 8 g/kg) has the best effect .
