世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
World Journal of Integrated Traditional and Western Medicine
2015年
11期
1529-1533
,共5页
黄芪%红花%最佳配比%酵母 α - 葡萄糖苷酶%抗氧化活性
黃芪%紅花%最佳配比%酵母 α - 葡萄糖苷酶%抗氧化活性
황기%홍화%최가배비%효모 α - 포도당감매%항양화활성
Radix Astragalus%Flos Carthami%Optimum Proportion%Alpha - Glucosidase%Antioxi-dant Activity
目的:依照国家食品药品监督管理总局(CFDA)制定的黄芪注射液与红花注射液的制备方法,分别对黄芪与红花进行相关提取。研究黄芪、红花提取物抑制α-葡萄糖苷酶及抗氧化活性的最佳配比。方法液相色谱仪测定黄芪中黄芪甲苷的含量,以山奈酚为标准品测定红花中总黄酮的含量。以4-甲基-伞形酮-β- D -半乳糖苷(4- MUG)为反应底物,测定样品对酵母α-葡萄糖苷酶活性的影响,阿卡波糖为阳性对照。以氧自由基清除能( Oxygen Radical Absorbance Capacity, ORAC)法测定样品的抗氧化活性,维生素 C 为阳性对照。结果每1 ml 黄芪提取液中含有0.127 mg黄芪甲苷,每1 ml 红花提取液中含0.57 mg 总黄酮,均符合 CFDA 有关活性成分的含量要求。红花抑制α-葡萄糖苷酶活性的半数抑制浓度(IC50)为(32.8±5.7)μg/ ml,显著低于黄芪[(1686±810)μg/ ml,P <0.01];黄芪与红花生药比为10:1、5:1、2:1时的 IC50值均显著低于20:1时(P <0.01)。红花的 ORAC 值为(1090±161)μmol TE/ g,显著高于黄芪[(205±32)μmol TE/ g,P <0.01];生药比为5:1及2:1时的 ORAC 值分别显著高于20:1及 10:1时(P <0.01)。结论黄芪与红花均具有抑制酵母α-葡萄糖苷酶活性的作用及一定的抗氧化活性,在同时改善以上两项指标方面,生药比为5:1及2:1时较20:1时作用显著(P <0.01)。
目的:依照國傢食品藥品鑑督管理總跼(CFDA)製定的黃芪註射液與紅花註射液的製備方法,分彆對黃芪與紅花進行相關提取。研究黃芪、紅花提取物抑製α-葡萄糖苷酶及抗氧化活性的最佳配比。方法液相色譜儀測定黃芪中黃芪甲苷的含量,以山奈酚為標準品測定紅花中總黃酮的含量。以4-甲基-傘形酮-β- D -半乳糖苷(4- MUG)為反應底物,測定樣品對酵母α-葡萄糖苷酶活性的影響,阿卡波糖為暘性對照。以氧自由基清除能( Oxygen Radical Absorbance Capacity, ORAC)法測定樣品的抗氧化活性,維生素 C 為暘性對照。結果每1 ml 黃芪提取液中含有0.127 mg黃芪甲苷,每1 ml 紅花提取液中含0.57 mg 總黃酮,均符閤 CFDA 有關活性成分的含量要求。紅花抑製α-葡萄糖苷酶活性的半數抑製濃度(IC50)為(32.8±5.7)μg/ ml,顯著低于黃芪[(1686±810)μg/ ml,P <0.01];黃芪與紅花生藥比為10:1、5:1、2:1時的 IC50值均顯著低于20:1時(P <0.01)。紅花的 ORAC 值為(1090±161)μmol TE/ g,顯著高于黃芪[(205±32)μmol TE/ g,P <0.01];生藥比為5:1及2:1時的 ORAC 值分彆顯著高于20:1及 10:1時(P <0.01)。結論黃芪與紅花均具有抑製酵母α-葡萄糖苷酶活性的作用及一定的抗氧化活性,在同時改善以上兩項指標方麵,生藥比為5:1及2:1時較20:1時作用顯著(P <0.01)。
목적:의조국가식품약품감독관리총국(CFDA)제정적황기주사액여홍화주사액적제비방법,분별대황기여홍화진행상관제취。연구황기、홍화제취물억제α-포도당감매급항양화활성적최가배비。방법액상색보의측정황기중황기갑감적함량,이산내분위표준품측정홍화중총황동적함량。이4-갑기-산형동-β- D -반유당감(4- MUG)위반응저물,측정양품대효모α-포도당감매활성적영향,아잡파당위양성대조。이양자유기청제능( Oxygen Radical Absorbance Capacity, ORAC)법측정양품적항양화활성,유생소 C 위양성대조。결과매1 ml 황기제취액중함유0.127 mg황기갑감,매1 ml 홍화제취액중함0.57 mg 총황동,균부합 CFDA 유관활성성분적함량요구。홍화억제α-포도당감매활성적반수억제농도(IC50)위(32.8±5.7)μg/ ml,현저저우황기[(1686±810)μg/ ml,P <0.01];황기여홍화생약비위10:1、5:1、2:1시적 IC50치균현저저우20:1시(P <0.01)。홍화적 ORAC 치위(1090±161)μmol TE/ g,현저고우황기[(205±32)μmol TE/ g,P <0.01];생약비위5:1급2:1시적 ORAC 치분별현저고우20:1급 10:1시(P <0.01)。결론황기여홍화균구유억제효모α-포도당감매활성적작용급일정적항양화활성,재동시개선이상량항지표방면,생약비위5:1급2:1시교20:1시작용현저(P <0.01)。
Objective To prepare radix astragalus injection and flos carthami injection according to the method of the China Food and Drug Administration(CFDA)and extract them respectively so as to study the optimum proportion of them on alpha - glucosidase and antioxidant activities. Methods The liquid chro-matograph was used to determine the content of astragaloside in radix astragalus. Kaempferol was used as the standard to determine the content of general flavone in flos carthami. 4 - MUG was as the oligomer to deter-mine the impacts of the samples to yeast alpha glycosidase enzyme activity,in which,acarbose was taken as the positive control. Oxygen radial absorbance capacity(ORAC)was adopted to determine the antioxidant ac-tivity of samples,in which,vitamin C was taken as the positive control. Results The astragaloside 0. 127 mg contained in each 1 ml radix astragalus extraction and the general flavone 0. 57 mg in each 1 ml flos carthami extraction. All of them met the content requirement of CFDA - relevant activity component. IC50 of the inhibi-tion of flos carthami for alpha - glucosidase activity was(32. 8 ± 5. 7)μg/ ml,significantly lower than that of radix astragalus[(1686 ± 810)μg/ ml,P < 0. 01]. IC50 was lower significantly at the ratios of radix astragalus and flos carthami as 10: 1,5: 1 and 2: 1 as compared with that at the ratio of 20: 1(P <0. 01)separately. ORAC value of flos carthami was(1090 ± 161)μmol TE/ g,higher significantly than that of radix astragalus[(205 ± 32)μmol TE/ g,P <0. 01]. When the ratios of the crude herbal materials were 5: 1 and 2: 1,ORAC value was higher significantly than that at 20: 1 and 10: 1 respectively(P <0. 01). Conclusion Both radix astragalus and flos carthami act on the inhibition of yeast alpha - glucosidase activity and has a certain of antioxidant activity. While improving the above indexes,the actions of the crude herbal materials are significant at the ratio of 5:1 and 2: 1 as compared with those at 20: 1(P < 0. 01).